Abstract: Abstract：To construct pEGFP-Kallikrein eukaryotic expression vector which used green fluorescence protein as reportor gene, and transfect rabbit endothelial progenitor cells. The human tissue kallikrein（tHK）gene were amplified with PCR, and inserted into pEGFP-N3 vector. The rabbit endothelial progenitor cells(EPCs) were transfected with the formed plasmid by means of lipidosome, and the kallikrein-EGFP fused protein was visued directly with fluorensce microscope, and the expression of tHK was detected by RT-PCR(reverse transcription-PCR) and ELISA. Plasmids was formed correctly and detected by PCR meanwhile being sequenced, and expressed in the rabbit EPCs. The fused protein has actitivites of both kallikrein and EGFP. The tHK genetically modified EPCs could play a role in the treatment of artery injury and endothelial dysfunction.

Key words: Kallikrein, Endothelial progenitor cells, Artery injury, Vascular disease