Basic & Clinical Medicine ›› 2023, Vol. 43 ›› Issue (4): 524-531.doi: 10.16352/j.issn.1001-6325.2023.04.0524
• Invited Reviews: Basic Research of Reproduction • Previous Articles Next Articles
ZHANG Qiang, SU Wenhui*
Received:
2022-08-05
Revised:
2022-12-20
Online:
2023-04-05
Published:
2023-04-03
Contact:
*whsu@cmu.edu.cn
CLC Number:
ZHANG Qiang, SU Wenhui. Advances in research on RNA N-6-methyladenosine(m6A) modification related enzymes in mammalian spermatogenesis[J]. Basic & Clinical Medicine, 2023, 43(4): 524-531.
[1]Cole LA. Human male spermatogenesis[M]//Cole LA. Biology of life. Online; Academic Press, 2016: 135-141. [2]Roundtree IA, Evans ME, Pan T, et al. Dynamic rna modifications in gene expression regulation[J]. Cell, 2017, 169: 1187-1200. [3]Boccaletto P, Baginski B. Modomics: An operational guide to the use of the rna modification pathways database[J]. Methods Mol Biol, 2021, 2284: 481-505. [4]Desrosiers R, Friderici K, Rottman F. Identification of methylated nucleosides in messenger rna from novikoff hepatoma cells[J]. Proc Natl Acad Sci U S A, 1974, 71: 3971-3975. [5]Jia G, Fu Y, Zhao X, et al. N6-methyladenosine in nuclear rna is a major substrate of the obesity-associated fto[J]. Nat Chem Biol, 2011, 7: 885-887. [6]Lin Z, Hsu PJ, Xing X, et al. Mettl3-/mettl14-mediated mrna n(6)-methyladenosine modulates murine spermatogenesis[J]. Cell Res, 2017, 27: 1216-1230. [7]Wang P, Doxtader KA, Nam Y. Structural basis for cooperative function of mettl3 and mettl14 methyltransferases[J]. Mol Cell, 2016, 63: 306-317. [8]Xiao W, Adhikari S, Dahal U, et al. Nuclear m6a reader ythdc1 regulates mrna splicing[J]. Mol Cell, 2016, 61: 507-519. [9]Ping XL, Sun BF, Wang L, et al. Mammalian wtap is a regulatory subunit of the rna n6-methyladenosine methyltransferase[J]. Cell Research, 2014, 24: 177-189. [10]Meyer KD, Saletore Y, Zumbo P, et al. Comprehensive analysis of mrna methylation reveals enrichment in 3' utrs and near stop codons[J]. Cell, 2012, 149: 1635-1646. [11]Dominissini D, Moshitch-Moshkovitz S, Schwartz S, et al. Topology of the human and mouse m6a rna methylomes revealed by m6a-seq[J]. Nature, 2012, 485: 201-206. [12]Yue Y, Liu J, Cui X, et al. Virma mediates preferential m(6)a mrna methylation in 3'utr and near stop codon and associates with alternative polyadenylation[J]. Cell Discov, 2018, 4: 10. [13]Ruan H, Yang F, Deng L, et al. Human m(6)a-mrna and lncrna epitranscriptomic microarray reveal function of rna methylation in hemoglobin h-constant spring disease[J]. Sci Rep, 2021, 11: 20478. [14]Patil DP, Chen CK, Pickering BF, et al. M(6)a rna methylation promotes xist-mediated transcriptional repression[J]. Nature, 2016, 537: 369-373. [15]Zheng GQ, Dahl JA, Niu YM, et al. Alkbh5 is a mammalian rna demethylase that impacts rna metabolism and mouse fertility[J]. Mol Cell, 2013, 49: 18-29. [16]Mauer J, Sindelar M, Despic V, et al. Fto controls reversible m(6)am rna methylation during snrna biogenesis[J]. Nat Chem Biol, 2019, 15: 340-347. [17]Roundtree IA, Luo GZ, Zhang Z, et al. Ythdc1 mediates nuclear export of n(6)-methyladenosine methylated mrnas[J]. Elife, 2017, 6. e31311.doi: 10.7554/eLife.31311. [18]Hsu PJ, Zhu Y, Ma H, et al. Ythdc2 is an n(6)-methyladenosine binding protein that regulates mammalian spermatogenesis[J]. Cell Res, 2017, 27: 1115-1127. [19]Wang X, Zhao BS, Roundtree IA, et al. N(6)-methyladenosine modulates messenger rna translation efficiency[J]. Cell, 2015, 161: 1388-1399. [20]Wang X, Lu Z, Gomez A, et al. N6-methyladenosine-dependent regulation of messenger rna stability[J]. Nature, 2014, 505: 117-120. [21]Shi H, Wang X, Lu Z, et al. Ythdf3 facilitates translation and decay of n(6)-methyladenosine-modified rna[J]. Cell Res, 2017, 27: 315-328. [22]Li A, Chen YS, Ping XL, et al. Cytoplasmic m(6)a reader ythdf3 promotes mrna translation[J]. Cell Res, 2017, 27: 444-447. [23]Huang H, Weng H, Sun W, et al. Recognition of rna n(6)-methyladenosine by igf2bp proteins enhances mrna stability and translation[J]. Nat Cell Biol, 2018, 20: 285-295. [24]Meyer KD, Patil DP, Zhou J, et al. 5' utr m(6)a promotes cap-independent translation[J]. Cell, 2015, 163: 999-1010. [25]Alarcon CR, Goodarzi H, Lee H, et al. Hnrnpa2b1 is a mediator of m(6)a-dependent nuclear rna processing events[J]. Cell, 2015, 162: 1299-1308. [26]Liu N, Dai Q, Zheng G, et al. N(6)-methyladenosine-dependent rna structural switches regulate rna-protein interactions[J]. Nature, 2015, 518: 560-564. [27]Liu N, Zhou KI, Parisien M, et al. N6-methyladenosine alters rna structure to regulate binding of a low-complexity protein[J]. Nucleic Acids Res, 2017, 45: 6051-6063. [28]Fu Y, Dominissini D, Rechavi G, et al. Gene expression regulation mediated through reversible m6a rna methylation[J]. Nat Rev Genet, 2014, 15: 293-306. [29]Bi Z, Liu Y, Zhao Y, et al. A dynamic reversible rna n(6) -methyladenosine modification: Current status and perspectives[J]. J Cell Physiol, 2019, 234: 7948-7956. [30]Cao G, Li HB, Yin Z, et al. Recent advances in dynamic m6a rna modification[J]. Open Biol, 2016, 6: 160003. [31]Shi H, Wei J, He C. Where, when, and how: Context-dependent functions of rna methylation writers, readers, and erasers[J]. Mol Cell, 2019, 74: 640-650. [32]Zaccara S, Ries RJ, Jaffrey SR. Reading, writing and erasing mrna methylation[J]. Nat Rev Mol Cell Biol, 2019, 20: 608-624. [33]Adhikari S, Xiao W, Zhao YL, et al. M(6)a: Signaling for mrna splicing[J]. RNA Biol, 2016, 13: 756-759. [34]Lesbirel S, Wilson SA. The m(6)amethylase complex and mrna export[J]. Biochim Biophys Acta Gene Regul Mech, 2019, 1862: 319-328. [35]Frye M, Harada BT, Behm M, et al. Rna modifications modulate gene expression during development[J]. Science, 2018, 361: 1346-1349. [36]Lukassen S, Bosch E, Ekici AB, et al. Characterization of germ cell differentiation in the male mouse through single-cell rna sequencing[J]. Sci Rep, 2018, 8: 6521. [37]Kalucka J, de Rooij L, Goveia J, et al. Single-cell transcriptome atlas of murine endothelial cells[J]. Cell, 2020, 180: 764-779 e720. [38]Yu XW, Li TT, Du XM, et al. Single-cell rna sequencing reveals atlas of dairy goat testis cells[J]. Zool Res, 2021, 42: 401-405. [39]Wu Y, Guo T, Li J, et al. The transcriptional cell atlas of testis development in sheep at pre-sexual maturity[J]. Curr Issues Mol Biol, 2022, 44: 483-497. [40]Yang H, Ma J, Wan Z, et al. Characterization of sheep spermatogenesis through single-cell rna sequencing[J]. FASEB J, 2021, 35: e21187. [41]Zhang L, Li F, Lei P, et al. Single-cell rna-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis[J]. J Anim Sci Biotechnol, 2021, 12: 122. [42]Zhang L, Guo M, Liu Z, et al. Single-cell rna-seq analysis of testicular somatic cell development in pigs[J]. J Genet Genomics, 2022,49:1016-1028. [43]Lau X, Munusamy P, Ng MJ, et al. Single-cell rna sequencing of the cynomolgus macaque testis reveals conserved transcriptional profiles during mammalian spermatogenesis[J]. Dev Cell, 2020, 54: 548-566 e547. [44]Wang M, Liu X, Chang G, et al. Single-cell rna sequencing analysis reveals sequential cell fate transition during human spermatogenesis[J]. Cell Stem Cell, 2018, 23: 599-614 e594. [45]Sun X, Zhang J, Jia Y, et al. Characterization of m6a in mouse ovary and testis[J]. Clin Transl Med, 2020, 10: e141. [46]Zhao X, Lin Z, Fan Y, et al. Ythdf2 is essential for spermatogenesis and fertility by mediating a wave of transcriptional transition in spermatogenic cells[J]. Acta Biochim Biophys Sin (Shanghai), 2021, 53: 1702-1712. [47]Chen Y, Wang J, Xu D, et al. M(6)a mrna methylation regulates testosterone synthesis through modulating autophagy in leydig cells[J]. Autophagy, 2021, 17: 457-475. [48]Liu SH, Ma XY, Yue TT, et al. Transcriptome-wide m6a analysis provides novel insights into testicular development and spermatogenesis in xia-nan cattle[J]. Front Cell Dev Biol, 2021, 9: 791221. [49]Liu Z, Chen X, Zhang P, et al. Transcriptome-wide dynamics of m(6)a mrna methylation during porcine spermatogenesis[J]. Genomics Proteomics Bioinformatics, 2021, S1672-0229(21)00181-9. doi: 10.1016/j.gpb.2021.08.006. [50]Zhao TX, Wang JK, Shen LJ, et al. Increased m6a rna modification is related to the inhibition of the nrf2-mediated antioxidant response in di-(2-ethylhexyl) phthalate-induced prepubertal testicular injury[J]. Environ Pollut, 2020, 259: 113911. [51]Soumillon M, Necsulea A, Weier M, et al. Cellular source and mechanisms of high transcriptome complexity in the mammalian testis[J]. Cell Rep, 2013, 3: 2179-2190. [52]Yu Y, Fuscoe JC, Zhao C, et al. A rat rna-seq transcriptomic bodymap across 11 organs and 4 developmental stages[J]. Nat Commun, 2014, 5: 3230. [53]Zimmermann C, Stevant I, Borel C, et al. Research resource: The dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis[J]. Mol Endocrinol, 2015, 29: 627-642. [54]Schwartz S, Agarwala Sudeep D, Mumbach Maxwell R, et al. High-resolution mapping reveals a conserved, widespread, dynamic mrna methylation program in yeast meiosis[J]. Cell, 2013, 155: 1409-1421. [55]Xu K, Yang Y, Feng GH, et al. Mettl3-mediated m(6)a regulates spermatogonial differentiation and meiosis initiation[J]. Cell Res, 2017, 27: 1100-1114. [56]Lasman L, Krupalnik V, Viukov S, et al. Context-dependent functional compensation between ythdf m(6)a reader proteins[J]. Genes Dev, 2020, 34: 1373-1391. [57]Jia GX, Lin Z, Yan RG, et al. Wtap function in sertoli cells is essential for sustaining the spermatogonial stem cell niche[J]. Stem Cell Reports, 2020, 15: 968-982. [58]Huang Y, Yan J, Li Q, et al. Meclofenamic acid selectively inhibits fto demethylation of m6a over alkbh5[J]. Nucleic Acids Res, 2015, 43: 373-384. [59]Huang T, Guo J, Lv Y, et al. Meclofenamic acid represses spermatogonial proliferation through modulating m(6)a rna modification[J]. J Anim Sci Biotechnol, 2019, 10: 63. [60]Tang C, Klukovich R, Peng H, et al. Alkbh5-dependent m6a demethylation controls splicing and stability of long 3′-utr mrnas in male germ cells[J]. Proc Natl Acad Sci U S A, 2018, 115: E325-E333. [61]Hong S, Shen X, Luo C, et al. Comparative analysis of the testes from wild-type and alkbh5-knockout mice using single-cell rna sequencing[J]. G3 (Bethesda), 2022, 12:jkac130. doi: 10.1093/g3journal/jkac130 [62]Cheng CY, Mruk DD. The blood-testis barrier and its implications for male contraception[J]. Pharmacol Rev, 2012, 64: 16-64. [63]Kasowitz SD, Ma J, Anderson SJ, et al. Nuclear m6a reader ythdc1 regulates alternative polyadenylation and splicing during mouse oocyte development[J]. PLoS Genet, 2018, 14: e1007412. [64]Roundtree IA, He C. Nuclear m6a reader ythdc1 regulates mrna splicing[J]. Trends Genet, 2016, 32: 320-321. [65]Wojtas MN, Pandey RR, Mendel M, et al. Regulation of m(6)a transcripts by the 3′-->5′ rna helicase ythdc2 is essential for a successful meiotic program in the mammalian germline[J]. Mol Cell, 2017, 68: 374-387 e312. [66]Jain D, Puno MR, Meydan C, et al. Ketu mutant mice uncover an essential meiotic function for the ancient rna helicase ythdc2[J]. Elife, 2018, 7:e30919. doi: 10.7554/eLife.30919 [67]Bai G, Zhai X, Liu L, et al. The molecular charac-teristics in different procedures of spermatogenesis[J]. Gene, 2022, 826: 146405. [68]Tang Z, Li C, Kang B, et al. Gepia: A web server for cancer and normal gene expression profiling and interactive analyses[J]. Nucleic Acids Res, 2017, 45: W98-W102. [69]Yang Y, Huang W, Huang JT, et al. Increased n6-methyladenosine in human sperm rna as a risk factor for asthenozoospermia[J]. Sci Rep, 2016, 6: 24345. [70]Glazer CH, Bonde JP, Giwercman A, et al. Risk of diabetes according to male factor infertility: A register-based cohort study[J]. Hum Reprod, 2017, 32: 1474-1481. [71]Chen X, Lin Q, Wen J, et al. Whole genome bisulfite sequencing of human spermatozoa reveals differentially methylated patterns from type 2 diabetic patients[J]. J Diabetes Investig, 2020, 11: 856-864. [72]Landfors M, Nakken S, Fusser M, et al. Sequencing of fto and alkbh5 in men undergoing infertility work-up identifies an infertility-associated variant and two missense mutations[J]. Fertil Steril, 2016, 105: 1170-1179.e1175. |
[1] | ZHONG Lin, ZHONG Hongli, DAI Yujie, ZHANG Qinghua. Effect of heat shock transcription factor 5(Hsf5) knockdown on heat shock family in mouse Leydig cells and Sertoli cells [J]. Basic & Clinical Medicine, 2023, 43(4): 626-631. |
[2] | TANG Jielin, LIU Jun, ZOU Dingfeng, MIAO Shiying, SONG Wei, LI Kai. Effect of Pabpc6 knockout on spermatogenesis of male mice [J]. Basic & Clinical Medicine, 2023, 43(4): 560-567. |
[3] | JIANG Shuyi, SU Wenhui. Advances in the effect of NC1 domain of type Ⅳ collagen on spermatogenesis and blood-testis barrier [J]. Basic & Clinical Medicine, 2023, 43(4): 532-537. |
[4] | ZHONG Hong-li, XIAO Cheng-liang, SHI Xiang-rui, DAI Yu-jie, LIU Wei, ZHANG Qing-hua. Expression, activity measurement and structure prediction of spermatogenic regulator:recombinant human HSF5 [J]. Basic & Clinical Medicine, 2021, 41(9): 1323-1328. |
[5] | LI Kai, MANG Xin-yu, ZOU Ding-feng, LI Meng-zhen, MIAO Shi-ying, WANG Lin-fang, SONG Wei. Dynamic expression of RNA-binding proteins in spermatogenesis based on RNA-seq [J]. Basic & Clinical Medicine, 2021, 41(6): 825-830. |
[6] | LI Yan-rong, LIU Guang-wei, ZHAO Jing, LI Ya-jun. Effects of main compositions of Astragalus on estradiol synthesizing activing in TGF-β1-induced rat testicular Leydig cell line [J]. Basic & Clinical Medicine, 2021, 41(1): 68-71. |
[7] | LI Ya-hui, LI Kai, LUO Yan-yun, LI Meng-zhen, MANG Xin-yu, SONG Wei, YANG Tao. Construction and expression verification of mouse spermatogenic cell-specific expression vector AAV-Dazl-RFP-Flag [J]. Basic & Clinical Medicine, 2020, 40(6): 753-758. |
[8] | . Construction and characterization of QKI gene knockout GC1-spg cell strain with CRISPR/CAS9 [J]. Basic & Clinical Medicine, 2018, 38(5): 589-593. |
[9] | . Advances in research on anti-Mullerian tube hormone and male reproductive endocrine-related diseases [J]. Basic & Clinical Medicine, 2018, 38(3): 405-408. |
[10] | . High fat diet-induced elevation of blood glucose impairs the microvascular function of testis in mice [J]. Basic & Clinical Medicine, 2018, 38(2): 213-217. |
[11] | Hong-yun HE; Yi-hao DENG; Xin-wen YANG; Kai-ming YANG. Puerarin Reduces Ethanol-induced-Apoptosis of Spermatogenic Cells in Rat Testis [J]. Basic & Clinical Medicine, 2009, 29(12): 1291-1295. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||
京ICP备07012236号
Website Copyright © Basic & Clinical Medicine