Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (12): 1829-1834.doi: 10.16352/j.issn.1001-6325.2022.12.1829

• Original Articles • Previous Articles     Next Articles

Construction and functional verification of AP-2 gene family eukaryotic expression vector

MI Yue, JIANG Qi-wei, SHEN Yan-jie, ZHANG De-yu*, YE Qi-nong*   

  1. Department of Medical Molecular Biology,Institute of Biotechnology,Academy of Military Medical Sciences, Academy of Military Sciences,Beijing 100850,China
  • Received:2021-12-28 Revised:2022-05-26 Online:2022-12-05 Published:2022-11-23
  • Contact: *;

Abstract: Objective To construct the eukaryotic expression recombinant plasmids of activator protein 2(AP-2) family genes and to identify the effect of AP-2α, AP-2β and AP-2γ on proliferation and migration of HepG2 cell line. Methods Ap-2α,AP-2β and AP-2γ gene were amplified with human mammary cDNA library as template and ligated with pCDNA3.0-FLAG vector, respectively. The eukaryotic expression vector pCDNA3.0-FLAG-AP-2α/β/γ was constructed and transfected into HepG2 cells. CCK8 assay and clone formation assay were used to detect the effect of AP-2 family genes on HepG2 cell proliferation. Scratch assay was used to detect the effect of AP-2 family genes on HepG2 cell migration. Results The pCDNA3.0-FLAG-AP-2α/β/γ were successfully constructed and expressed in HepG2 cells.AP-2 family genes significantly inhibited the proliferation and migration of HepG2 cells(P<0.05). Conclusions Ap-2α, AP-2β and AP-2γ may inhibit the proliferation and migration of HepG2 cells.

Key words: human AP-2 family genes, hepatocellular carcinoma, eukaryotic expression, cell line HepG2

CLC Number: