Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (6): 969-974.doi: 10.16352/j.issn.1001-6325.2022.06.010

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Advantages, disadvantages and research progress on strategies for screening and identifying functional genes

LIU Xu1, CHANG De1*, WANG Jun-feng2   

  1. 1. the Third Medical Center,Chinese People's Liberation Army General Hospital, Beijing 100069;
    2. the Second Medical Center,Chinese People's Liberation Army General Hospital, Beijing 100853, China
  • Received:2020-09-07 Revised:2022-04-11 Online:2022-06-05 Published:2022-06-02
  • Contact: *

Abstract: The screening and identification of functional genes is a key step to deeply understand various biological phenotypes. Forward genetics including traditional genetics, genome-wide association analysis, the comparison of gene expression profiles and bioinformatics, and reverse genetics including cDNA library, RNA interference, CRISPR-Cas9 technology, insertion mutation as well as model organisms have been applied to screening functional genes. Different methods have their own advantages and disadvantages and technology combination to screen and to identify functional genes is a good choice of strategy.

Key words: functional genes, forward genetics, reverse genetics, bioinformatics, model organism

CLC Number: