Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (3): 472-478.doi: 10.16352/j.issn.1001-6325.2022.03.020

• Original Articles • Previous Articles     Next Articles

Circ-MALAT1 targeting miR-101 inhibits invasion and migration of bladder cancer cell line SW780

YANG Mang-zhuang*, YANG Xu-dong, WANG Xiao-long   

  1. Department of Urology, Yellow River Sanmenxia Hospital,Sanmenxia 472000, China
  • Received:2021-01-22 Revised:2021-07-02 Online:2022-03-05 Published:2022-03-04
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Abstract: Objective To explore the function of circ-MALAT1 targeting miR-101 in the inhibition of the invasion and migration of bladder cancer cells. Methods Bladder cancer cell line SW780 was divided into si-NC group (transfection si-NC), si-circ-MALAT1 group (transfection si-circ-MALAT1), miR-NC group (transfection miR-NC), miR-101 group (transfection miR-101 mimics), si-circ-MALAT1+anti-miR-NC group(co-transfection si-circ-MALAT1 and anti-miR-NC)and si-circ-MALAT1+anti-miR-101 group(co-transfection si-circ-MALAT1 and anti-miR-101). RT-qPCR was used to examine the expression of circ-MALAT1 and miR-101; Transwell method was used to detect cell invasion and migration; The matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9),phosphorylated phosphatidylinositol 3-kinase (p-PI3K), phosphorylated protein kinase B(p-AKT) protein expression were measured by Western blot; Dual luciferase reporter gene experiment was applied to detect the targeting relationship between circ-MALAT1 and miR-101. Results Compared with adjacent tissues, the expression of circ-MALAT1 in bladder cancer tissue was significantly increased (P<0.05)and the expression of miR-101 was significantly decreased (P<0.05). Compared with the si-NC group, the expression of circ-MALAT1 in the si-circ-MALAT1 group was significantly reduced(P<0.05)and the counting number of invasion and migrating cells was significantly reduced (P<0.05), the expression of p-PI3K and p-AKT protein was significantly reduced(P<0.05). Compared with the miR-NC group, the expression of miR-101 in the miR-101 group was significantly increased(P<0.05)and the number of invasion as well as migrating cells were significantly reduced(P<0.05).Compared with the (si-circ-MALAT1+anti-miR-NC)group, the(si-circ-MALAT1+anti-miR-101)group showed more cell invasion and migration (P<0.05), the expression of p-PI3K and p-AKT protein was significantly increased(P<0.05). Conclusions Reduction of circ-MALAT1 expression and up-regulation miR-101 inhibit the invasion and migration of bladder cancer cells line SW780. The mechanism may be related to the PI3K/AKT signaling pathway.

Key words: circ-MALAT1, miR-101, bladder cancer, PI3K/AKT signaling pathway, invasion

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