Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (3): 305-309.

• Original Articles • Previous Articles     Next Articles

Estrogen promotes proliferation and invasion of astrocyte cell line U-87

LIU Yan-min1,2, FEI Yi-lin1, LI Xing-miao1, WANG Ning1, CHEN Mo1, JIANG Xiu-xiu1*   

  1. 1.Department of Gynecology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006;
    2. Department of Obstetrics and Gynecology, Central Hospital of Haining, Haining 314408, China
  • Received:2019-04-18 Revised:2019-09-24 Online:2020-03-05 Published:2020-03-02
  • Contact: *

Abstract: Objective To explore the relationships between the estrogen and astrocyte cell proliferation as well as invasion. Methods Astrocyte cell line U-87 was cultured in vitro and treated with 0, 10-12, 10-10, 10-8 and 10-6 mol/L estrogen. The gene and protein expressions were analyzed by qPCR, Western blot, ELISA; and the cell proliferation and invasion was analyzed by CCK-8 kits and transwell experiments. Results Estrogen significantly inhibited IL-1, TNF-α, IL-6 expression and promoted the expression of NGF and PGE2 (P<0.05). Furthermore, estrogen inhibited aquaporin 2(AQP2) expression (P<0.05). The proliferation and invasion experiments showed that estrogen promoted the U-87 cell proliferation and invasion (P<0.05). Conclusions Estrogen down-regulates the expression of inflammatory factors, inhibites the expression of AQP2, promotes the proliferation and invasion of U-87 cells.

Key words: estrogen, astrocyte, aquaporin 2, proliferation

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