Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (3): 315-320.

• Original Articles • Previous Articles     Next Articles

Triptolide inhibits the proliferation and promotes apoptosis of non-small cell lung cancer cell line A549

LIU Qian1*, TANG Jian-hua1, ZHANG Zhi-hua2   

  1. 1. Pharmacy Department;
    2. Department of Respiratory Medicine, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China
  • Received:2019-03-26 Revised:2019-09-30 Online:2020-03-05 Published:2020-03-02
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Abstract: Objective To investigate the effect of triptolide (TP) on the proliferation and apoptosis of human non-small cell lung cancer cell A549 by regulating the expression of chemokine receptor 4 (CXCR4) gene. Methods The experiment was divided into 4 groups: control group, TP group (100 nm/L TP-treated cells), CXCR4+TP group (transfected plasmid TP-treated cells) and NC+TP group (empty plasmid TP-treated cells). The expression of CXCR4 and transfection were detected by RT-qPCR and Western blot. Cell proliferation was detected by MTT assay, apoptosis was detected by Annexin V-FITC/PI double staining, proliferation and apoptosis-related protein expression were detected by Western blot. Results Triptolide inhibited the expression of CXCR4 mRNA and protein in A549 cells (P<0.05). Triptolide inhibited the proliferation of A549 cells and induced apoptosis(P<0.05). Transfection of pcDNA-CXCR4 up-regulated the expression of CXCR4 mRNA and protein (P<0.05). Up-regulation of CXCR4 expression partially reversed the effect of triptolide on proliferation inhibition and apoptosis induction of A549 cells (P<0.05). Conclusions Triptolide may inhibit the proliferation of non-small cell lung cancer A549 cells and induce apoptosis by down-regulating the expression of CXCR4.

Key words: triptolide, CXCR4 gene, non-small cell lung cancer cell line A549, proliferation, apoptosis

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