Basic & Clinical Medicine ›› 2016, Vol. 36 ›› Issue (12): 1687-1692.
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Abstract: Objective To investigate the protective effect and mechanism of matrix metalloproteinase inhibitor-1 (TIMP-1) against diabetic retinopathy in mice. Methods A total of 96 of 6-8 weeks old C57bl mice were randomly divided into 3 groups: control group, diabetic group and TIMP-1 group (mice were intravitreously injected with TIMP-1 5μl (1μg/ml) in the first and second months respectively after diabetes-induced). Retinal vascular leakages were investigated by retinal perfusion of evans blue after 5 months. The mRNA and protein expression levels of metalloproteinase 9 (MMP-9), vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNF-α) and epoxy synthase -2 (COX-2) were also analyzed by RT-qPCR and Western blot. And the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity and malondialdehyde (MDA) content in the retina tissue were also detected with bio-chemical method. Results There was no retinal leakage in control group, but diabetic group was significantly higher than TIMP-1 group (p<0.01). The mRNA and protein expression levels of MMP-9, VEGF, TNF-α, COX-2 and the MDA content in diabetic group were significantly higher than control group (p<0.01), while in TIMP-1 group they decreased (p<0.01) and also higher than control group (p<0.01). The SOD and GSH-Px activity in diabetic group were significantly lower than control group(p<0.01),while in TIMP-1group they increased (p<0.01) and also lower than control group (p<0.01). Conclusions TIMP-1 can protect the retina of diabetic mice by reducing the oxidative stress and the expression of VEGF.
Key words: diabetic retinopathy, matrix metalloproteinase inhibitor-1, oxidative stress
CLC Number: (Dielectrics, piezoelectrics, and ferroelectrics and their properties)
77 (Dielectrics, piezoelectrics, and ferroelectrics and their properties)
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2016/V36/I12/1687