Basic & Clinical Medicine ›› 2015, Vol. 35 ›› Issue (7): 925-928.
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Abstract: Objective: To investigate the influence of over-expressed S100A6 on proliferation,migration and apoptosis in eutopic endometrial stromal cells. Methods: The eutopic endometrial stromal cells were cultivated in vitro and divided into Lv-S100A6 group and negative group, which infected with the recombinant lentivirus S100A6 (Lv-S100A6) and recombinant lentivirus vector respectively. CCK-8 assay was performed to identify the proliferation of cell. The cell migration was detected by Transwell chamber. Flow cytometry method was performed to detect cell apoptosis. Results: As compared with the blank group and negative control group, the proliferation was increased in eutopic endometrial stromal cells after transfection with Lv-S100A6 (P<0.05), the ability of migration was promoted. However, the apoptosis rate in eutopic endometrial stromal cells after transfection with Lv-S100A6(2.99%)was decreased compared with the blank control group(13.48%) and the negative group(14.40%)(P<0.05). Conclusions: S100A6 can effectively increase the proliferation and migration in eutopic endometrial stromal cells, and inhibit the cell apoptosis.
Key words: S100A6, endometriosis, proliferation, migration, apoptosis
CLC Number:
R711.71
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URL: http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/
http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2015/V35/I7/925