Basic & Clinical Medicine ›› 2013, Vol. 33 ›› Issue (9): 1123-1128.
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Abstract: Objective:To Investgiate the expression of C/EBPβ in APL cells during differentiation induced by TanⅡA. Methods TanⅡA treated NB4 and MR2 cells 120h, the characterizations of differentiation were examined by morphology and membrane differentiation antigens;The mRNA and protein expressions of C/EBPβ in different concentration TanⅡA-intervened APL cells were examined by RT-PCR and Western-blot;APL cells were treated with different concentration TanⅡA, the characterizations of differentiation were examined by membrane differentiation antigens. Results TanIIA induced the differentiation of ATRA-sensitive and ATRA-resistant APL cells(p<0.05); TanIIA dose-dependend upregulated the expression of C/EBPβin mRNA and protein(p<0.05); TanIIA promoted the differentiation of the NB4 and MR2 cells in a dose-independent manner and exerted its strongest effect at a concentration of 1 mg/L. Conclusion TanIIA promoted the differentiation of ATRA-sensitive and ATRA-resistant APL cells and exerted its strongest effect at a concentration of 1mg/L; C/EBPβ played a key role in TanIIA-induced differentiation.
Key words: TanⅡA, C/EBPβ, differentiation
CLC Number:
R557
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2013/V33/I9/1123