Abstract: Abstract: Objective To investigate protein and gene dynamic expressions of leptin, collagen I and collagen III in the tissues of hepatic fibrosis model. Methods Liver fibrosis models of rats were made by hypodermic injection with 60% CCl4. The protein and gene expressions of leptin, collagen I and collagen III were assayed by Western blot and reverse transcription polymerase chain reaction(RT-PCR), respectively. Results The protein and gene expressions of leptin, collagen I and collagen III were slight in normal liver tissues. In the model groups, the expressions began to increase progressively at the end of the 2nd week after injection with CCl4. The mRNA expression levels of leptin, collagen I and collagen III in the model groups were higher than that in the control group (P<0.05), and there were closely positive correlation between leptin and collagen I(r=0.595, P=0.017), collagen III (r=0.478, P=0.011).Conclusion In the process of hepatic fibrosis induced by CCl4 injection in rats, expression of leptin and collagen I, III are increased with the development of hepatic fibrosis. Leptin probably participate the synthesis and degradation of extracellular matrix in hepatic fibrosis.

Key words: Hepatic fibrosis, Leptin, Collagen I, Collagen III