Basic & Clinical Medicine ›› 2012, Vol. 32 ›› Issue (7): 783-787.
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1,Bin YIN2,Xiao-zhong PENG
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Abstract: Abstract:Objective To investigate the influence of overexpression of miR-195 on cell proliferation in glioma T98G cells and explore the function of miR-195 in glioma. Method T98G cells containing lower expression level of miR-195 were transfected with synthesized miR-195 mimics , then MTT assay, flow cytometry and TUNEL assay were performed to analysis the alterations of cellular phenotype,western blot was performed to examine the expression level of BCL-2. Result Mature mir-195 increased significantly after miR-195 mimics transfection (P<0.05). Overexpression of miR-195 induced significant cell proliferation suppression. Flow cytometry results showed that up-regulated miR-195 produced little influence on cell cycle, while TUNEL assay indicated that miR-195 promoted apoptosis in T98G cells. Western blot result showed that BCL-2 was reduced after by miR-195 mimics. Conclusion Overexpression of miR-195 significantly promoted cell apoptosis in T98G cells, suggesting that miR-195 may function in glioma treatment.
Key words: miR-195, T98G cells, cell proliferation, apoptosis, BCL-2
CLC Number:
Q291
Bin YIN Xiao-zhong PENG. Overexpression of miR-195 promotes apoptosis in glioma T98G cells[J]. Basic & Clinical Medicine, 2012, 32(7): 783-787.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2012/V32/I7/783