Basic & Clinical Medicine ›› 2012, Vol. 32 ›› Issue (2): 175-180.

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Mechanism of PLCepsilon gene blockage on invasive power of human bladder cancer cells

Li-Ping OU1,Chun-Li LUO   

  • Received:2010-12-31 Revised:2011-08-22 Online:2012-02-05 Published:2012-01-12
  • Contact: Chun-Li LUO
  • Supported by:
    Research Item Foundation of Educational Committee in Chongqing

Abstract: Objective The aim of this study was to construct a shRNA expression plasmid against gene PLCε and to observe the inhibition of PLCε gene expression in bladder cancer cell line T24.Methods pGenesil-PLCε plasmids were constructed and insert T24 cells,then RT-PCR, Western blot analysis was taken to know about inhibition of PLCε gene expression after the transfection of plasmids. invasive power of T24 were measured before and after transfection by the membrane invasion culture system(Transwell chamber), gelatin enzymography and immunochemistry of cells. Results It was confirmed by digesting and sequencing that the two recombinant plasmids had been constructed successfully. The inhibition rate of PLCε mRNA was 76.0% and 73.9%, and this of protein expression was 65.4% and 64.8% . The invasion number of T24 transfected with P1(25.8±6.2) and P2(26.8±5.8) are both lower than the the group of HK-A (33.8±5.7 )and group without transfection(34.8±6.9) (P<0.01); MMP-2,MMP-9 had lower expression in gelatin enzymography assay and imunocytochemistry of cells than the groups HK-A and control(p<0.01). Conclusion:RNAi of PLCε might decrease invasive power of bladder cancer cells so as to inhibit the development of tumor.

Key words: Bladder Cancer, invasive power, PLCε

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