Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (3): 411-416.doi: 10.16352/j.issn.1001-6325.2022.03.029

• Original Articles • Previous Articles     Next Articles

Sulforaphane alleviates ischemia- reperfusion injury of heart transplantation in rat models

WANG Shuai1*, WANG Jian-jun2, SUN Xiu-hong1, WU Peng-yu1, CUI Zhi-cheng1, LI Zhan-qing1   

  1. 1. Department of Cardiovascular Surgery;
    2. Department of Critical Care Medicine(ICU), the Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,China
  • Received:2020-10-15 Revised:2021-07-02 Online:2022-03-05 Published:2022-03-04
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Abstract: Objective To investigate the effect of sulforaphane on ischemia-reperfusion injury(I/RI) and the role of PI3K/Akt/GSK-3β pathway in heart transplantation of rats. Methods The rat model of allograft heart transplantation was established as the control group.The survival rate,the level of serum myocardial enzymes,apoptosis of myocardial cells and the swelling degree of myocardial mitochondria were compared with sulforaphane injection group (2.5 mg/kg).Western blot was used to detect the expression of apoptotic molecules caspase-3,Bax and PI3K/Akt/GSK-3β. Results The survival rate of sulforaphane group was significantly higher than that of control group(P<0.05).The serum concentrations of LDH,CK,CK-MB and TNT in sulforaphane group were lower than those in control group with a time dependent manner at different treatment time (6,12,24 and 48 h).The expressions of caspase-3 and Bax were significantly decreased (P<0.01),and the degree of mitochondrial swelling was also improved compared with the control group. Conclusions Sulforaphane may inhibit myocardial apoptosis by activating PI3K/Akt/GSK-3β pathway and improve I/RI after heart transplantation as shown by animal model.

Key words: sulforaphane, heart transplantation, apoptosis, ischemia-reperfusion injury

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