Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (2): 282-285.doi: 10.16352/j.issn.1001-6325.2022.02.007

• Original Articles • Previous Articles     Next Articles

Hepatocyte-specific Sirt1 deficiency exacerbates liver inflammation of mice

YANG Jia-hui1, ZHAO Wei2, XIE Xiang-hong2, LI Chun-mei2, LIU Xiao-jun2, YAO Hong1*   

  1. 1. Department of Microbiology and Immunology, Basic Medicine College, Shanxi Medical University, Taiyuan 030001;
    2. National Key Laboratory of Medical Molecular Biology, Insititute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China
  • Received:2021-10-14 Revised:2021-12-06 Online:2022-02-05 Published:2022-01-24
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Abstract: Objective To investigate the effect of deletion of Sirtuin type 1(Sirt1) gene on liver inflammation of mice and its mechanism. Methods The body weight of C57BL/6J male mice and liver specific Sirt1 deficiency (Sirt1-LKO) male mice were recorded weekly. The sensitivity of inflammatory response was tested by intraperitoneal injection of 20 mg/kg LPS. The survival curve was used to reflect the sensitivity of mice to inflammatory response.The expression of inflammatory factors was detected by Western blot and q-PCR. Results Compared with control wild type mice, Sirt1-LKO mice showed significantly reduced body weight (P<0.05). In addition, The survival curve was shown by kaplane Meier diagram,Sirt1-LKO mice showed lower sensitivity to LPS (P<0.05).The protein level of NF-κB in primary liver cells of Sirt1-LKO mice was increased (P<0.05),Tnfα and IL6 of NF-κB downstream target genes were up-regulated(P<0.05). Conclusions Hepatocyte-specific Sirt1 deletion aggravates liver inflammation response in mouse model.

Key words: Sirt1, hepatocyte inflammation response, NF-κB

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