Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (11): 1656-1660.doi: 10.16352/j.issn.1001-6325.2022.11.1656

• Original Articles • Previous Articles     Next Articles

Effect of calcium channel blocker on steroid hormone profile of primary cultured aldosterone producing adenoma cells

GAO Yin-jie1, YU Song-lin2, YIN Yi-cong2, CUI Yun-ying1, ZHANG Yu-shi3, QIU Ling2, TONG An-li1*   

  1. 1. Department of Endocrinology,Key laboratory of Endocrinology of National Health Commission;
    2. Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases;
    3. Department of Urology, Peking Union Medical College Hospital, CAMS & PUMC, Beijing 100730, China
  • Received:2022-05-05 Revised:2022-06-29 Online:2022-11-05 Published:2022-11-01
  • Contact: *

Abstract: Objective To explore the influence of calcium channel blocker(CCB), verapamil, on steroid hormone profile of primary cultured aldosterone producing adenoma(APA)cells. Methods The adrenal tumor tissues of 6 APA patients with hypokalemia operated in Peking Union Medical College Hospital were cultured and treated with different concentrations of verapamil. The steroid hormone profile was detected by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Results The level of aldosterone in 50 μmol/L verapamil group was lower than that in 10 μmol/L verapamil group and control group(P<0.01). The levels of aldosterone precursor, 11-deoxycorticosterone in verapamil 10 μmol/L group was lower than that in control group (P<0.05). The levels of 18-oxocortisol(18-OXOF) and 18-hydroxycortisol(18-OHF) in verapamil 50 μmol/L group and verapamil 10 μmol/L group were lower than that in the control group (P<0.05). In addition, verapamil also had some inhibitory effects on glucocorticoids such as cortisol and cortisone, and adrenal derived sex hormones such as dehydroepiandrosterone sulfate(DHEA-S) and 11 beta-hydroxytestosterone(11β-OHT). Conclusions In addition to inhibit-ing aldosterone secretion in primary cultured APA cells, CCB has significant inhibitory effects on a variety of other steroid hormones, such as precursor 11-deoxycorticosterone, cortisol, metabolites, 18-OXOF and 18-OHF.

Key words: aldosterone producing adenoma, steroid hormone profile, aldosterone, 18-oxocortisol, 18-hydroxycortisol

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