Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (1): 94-99.

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Specific necroptosis inhibitor-1 (Nec-1)attenuates glial scar formation in rat models with spinal cord injury

  

  • Received:2020-11-09 Revised:2021-05-07 Online:2022-01-05 Published:2022-01-05

Abstract: Objective: To observe the effect of necroptosis inhibitor-1 (Nec-1) on glial scar formation in rats with spinal cord injury and explore its mechanism. Methods: The rats were randomly divided into sham operated group, model group and RIP1 K inhibitor Nec-1 group. After the spinal cord injury model group was successfully constructed, 0.9% sodium chloride solution and 10 μmol/L Nec-1 were injected into the lateral ventricles of the rats, respectively. In the sham operation group, rats only were opened the lamina and did not hit the spinal cord. At 1st,3rd,7th,14th day after operation, the motor function of the hind limbs was measured by BBB, the formation of glial scar was detected by immunofluorescence, and the expressions of CathepsinB, Caspase-3, GFAP and vimentin were detected by Western blot. Results: On the 14th day after operation, compared with the sham operation group, the BBB scores of the model group and Nec-1 group were significantly lower (P < 0.05), compared with the model group, the BBB score of Nec-1 group was significantly higher (P < 0.05). On the 14th day after operation, NF-200 fluorescence was not found in the sham operation group, but in the model group and Nec-1 group, compared with the model group, the intensity of NF-200 fluorescence labeling in Nec-1 group decreased significantly (P < 0.05). On the 7th and 14th day after operation, compared with the sham operation group, the protein levels of CathepsinB, Caspase-3, GFAP and vimentin in the model group and Nec-1 group were significantly higher (P < 0.05), compared with the model group, the protein levels of CathepsinB, Caspase-3, GFAP and vimentin in Nec-1 group decreased significantly (P < 0.05). Conclusions: Nec-1 may regulate CathepsinB-Caspase pathway, reduce the expressions of GFAP and vimentin proteins, reduce the formation of glial scar after spinal cord injury, and promote the recovery of neural function.