Basic & Clinical Medicine ›› 2022, Vol. 42 ›› Issue (1): 68-74.

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LncRNA expression profile in human malignant embryonic rhabdomyosarcoma cell line infected with Coxsackie virus group B type 5


  • Received:2021-05-18 Revised:2021-11-03 Online:2022-01-05 Published:2022-01-05
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Abstract: Objective: To explore the possible molecular mechanism of the interaction between Coxsackie virus Coxsackie virus group B type 5 (CVB5) and the host, the long non-coding RNA expression profile in human prototypic embryonal rhabdomyosarcoma cell line (RD) infected by CVB5 was investigated. Methods After 24 hours of RD cells infected with 1MOI CVB5 and extracted for RNA samples, and RNA-seq technology was used to obtain the differential expression profile of lncRNA in the cells, differentially expressed transcripts were analyzed by cluster analysis, GO analysis and KEGG pathway enrichment. At the same time, RNAfold was used to predict the secondary structure of lncRNA. Rusults Compared to the uninfected group, 1 754 mRNAs and 508 lncRNAs were up-regulated, and 3 106 mRNAs and 760 lncRNAs were down-regulated after CVB5 infection of RD cells. Co-expressed genes that differentially express lncRNA are mainly enriched in biological processes such as molecular structure activity, protein molecular binding, and humoral immune response; lncRNA targets are mainly involved in olfactory transduction pathway, cytokine-cytokine receptors interaction, neuroactive ligand-receptor interaction and other pathways. In addition, the seven differentially expressed lncRNAs verified by quantitative real-time PCR (RT-qPCR) are consistent with the sequencing results. Conclusions LncRNA is mainly involved in the regulation of the immune processes, which lays the foundation for a full understanding of the regulatory role of lncRNA in CVB5 infection .

Key words: RNA-seq, Coxsackie virus B5, long non-coding RNA, RD cells, expression profile