Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (7): 1018-1023.

• Original Articles • Previous Articles     Next Articles

Anlotinib combined with chloroquine induces apoptosis of human non-small cell lung cancer cell line H1299

LIU Xi-bang1, ZHU Lin-zhi1, JIAO De-min2, TANG Xia-li2, CHEN Jun2, HU Xu-gang2, CHEN Qing-yong1*   

  1. 1. The 1st School of Medical, Wenzhou Medical University, Wenzhou 325000;
    2. Department of Pulmonary and Critical Care Medicine, the 903 Hospital of the Chinese People's Liberation Army Joint Logistic Support Force, Hangzhou 310000,China
  • Received:2020-07-24 Revised:2020-11-19 Online:2021-07-05 Published:2021-06-17
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Abstract: Objective To investigate the effects of anlotinib and chloroquine (CQ) on viability, invasion and apoptosis of H1299 cells. Methods After treatment of H1299 cells with anlotinib or/and CQ, the cell viability was measured by MTT assay; Wound-healing and Transwell chamber assay were used to evaluate cell migration and invasion abilities; Hoechst 33342 staining was adopted to test the changes of apoptosis; The protein levels of autophagy marker LC3-Ⅱ/LC3-Ⅰ and autophagic substrate p62 were detected by Western blot. Results Anlotinib combined with CQ showed more obviously synergistic inhibitory effect on the viability, invasion and migration in H1299 cells than anlotinib alone (P<0.05). As compared to the untreated control cells, anlotinib alone promoted the cell apoptosis. Their combination induced an even higher apoptosis in H1299 cells. With the increase of concentration, anlotinib significantly up-regulated autophagy marker protein LC3-Ⅱ/LC3-Ⅰ and down-regulated autophagic substrate protein p62.The expression of LC3-Ⅱ/LC3-Ⅰ and p62 was significantly up-regulated by CQ. Compared with the CQ group alone, LC3-Ⅱ/LC3-Ⅰ and p62 were significantly up-regulated and down-regulated in the 20 μmol/L anlotinib combined with CQ group. Conclusions Combination of anlotinib and CQ remarkably inhibits the viability, migration and invasion and induces the apoptosis of H1299 cells. The potential mechanism for antitumor might be due to autophagy's action.

Key words: non-small cell lung cancer(NSCLC), anlotinib, chloroquine, autophagy, apoptosis

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