Long non-coding RNA MCM3AP-AS1 targeted miR-876-5p promotes the proliferation and migration of colorectal cancer cell lines

Abstract

Abstract: Objective To investigate the effect and mechanism of long non-coding RNA MCM3AP antisense transcript 1 and miR-876-5p on the proliferation and migration of colorectal cancer(CRC) cells in vitro. Methods The expressions of MCM3AP-AS1 in CRC tissue and cells were detected by real time quantitative polymerase chain reaction (RT-qPCR). The proliferation and migration of CRC cells were detected by MTT, scratch test and Transwell assay; and the targeting relationship between MCM3AP-AS1 and miR-876-5p was verified by bioinformatics analysis, luciferase assay and RT-qPCR. Results Compared with normal adjacent tissues and normal CRC epithelial cell lines, the expression of MCM3AP-AS1 was up-regulated. Down-regulation of MCM3AP-AS1 decreased the proliferation and migration of colorectal cancer cells. MCM3AP-AS1 targeted at miR-876-5p and down-regulated its expression. miR-876-5p inhibited the promotion of malignant phenotype of colon cancer. Conclusions MCM3AP-AS1 may promote the proliferation and migration of CRC cancer cells by absorbing of miR-876-5p.

Key words: MCM3AP-AS1, miR-876-5p, colorectal cancer, proliferation, migration

CLC Number:

R735

ZHOU Di-xia, LYU Hai-dong. Long non-coding RNA MCM3AP-AS1 targeted miR-876-5p promotes the proliferation and migration of colorectal cancer cell lines[J]. Basic & Clinical Medicine, 2021, 41(2): 225-232.

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