Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (2): 209-213.

• Original Articles • Previous Articles     Next Articles

Osteogenic activity of polyetheretherketone/58S bioactive glasses bone implant

LIU Lyu-hua1, ZHENG Yan-yan1*, ZHANG Li-fang2, XIONG Cheng-dong2   

  1. 1. School of Basic Medical Sciences, North Sichuan Medical College, Nanchong 637000;
    2. Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, China
  • Received:2020-06-27 Revised:2020-08-24 Online:2021-02-05 Published:2021-01-19
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Abstract: Objective To explore the osteogenic activity of polyetheretherketone/58S bioactive glasses (PEEK/58S) as a kind of bone implant. Methods MG-63 osteoblasts were cultured on PEEK and PEEK/58S surfaces for predetermined time. Then MG-63 osteoblast attachment and spreading condition were observed by scanning electron microscopy (SEM). The proliferation activity of MG-63 osteoblast was evaluated by CCK-8 (cell counting kit). Alkaline phosphatase (ALP) activity was measured to determine the osteogenic differentiation of MG-63 osteoblast. The mRNA expression levels of osteogenesis-related genes ALP, BMP-2, COL-I, OCN and OPN were detected using real-time fluorescent quantitative PCR. Osseointegration of PEEK and PEEK/58S bone implant was evaluated by histological analysis and biomechanical pull-out tests after implantation in a rat tibia model for 12 weeks. Results Compared with the PEEK, the adhesion and spreading of MG-63 osteoblast was more favorable. The proliferation activity and ALP activity of MG-63 osteoblast was significantly promoted (P<0.05). Also, the mRNA expression of ALP, BMP-2, COL-I, OCN and OPN was significantly up-regulated (P<0.05). In vivo experiments showed that PEEK/58S exhibited better osseointegration than pure PEEK. Conclusions PEEK/58S bone implant significantly improves the attachment, spreading, proliferation and differentiation of osteoblast and enhances the peri-implant osseointegration, so it is a potential novel orthopedic material for clinical use.

Key words: polyetheretherketone, 58S bioactive glasses, bioactivity, osteogenic activity, osseointegration

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