Abstract: Objective To study the protective effect of Ziziphi Spinosae Semen Flavonoids (ZSSF) on liver oxidative damage induced by perfluorooctane sulfonate (PFOS) in mice. Methods Mice were divided into blank control group, model group, positive control group (vitamin C group) and high, medium and low dose ZSSF group by random number table method, with 10 mice in each group. At the same time, PFOS (10 mg/kg) was molded and administered continuously for 30 days.After 6 h after the last delivery, eyeball blood in mice, the liver to join 9 times the volume of 0.9% sodium chloride solution in homogenate is made from 10% of the tissue of slurry, according to the kit instruction alanine transaminase (ALT) in serum and aspirate aminotransferase (AST), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (gsh-px) and catalase (CAT) and total antioxidant capacity (T -AOC) level; The expression of Nrf2 and HO-1 mrna in liver tissues was detected, and real-time fluorescent quantitative PCR(RT-qPCR) was applied. Results Compared with the control group, the contents of alanine aminotransferase, aspartate aminotransferase, and malondialdehyde increased in the model group, while the contents of superoxide dismutase, glutathione peroxidase, catalase and total antioxidant capacity decreased(P＜ 0.01, P＜0.05); Compared with the model group, the drug intervention group reduced the content of AST, ALT and MDA, and increased the content of SOD, CAT, GSH-Px, T-AOC, Nrf2 and HO-1 mRNA in liver tissue(P＜0.01, P＜0.05). Conclusions Ziziphi Spinosae Semen Flavonoids can improve the oxidative liver injury induced by PFOS in mice, which may be caused by increasing the activity of antioxidant enzymes in vivo and reducing the generation of lipid peroxides by regulating the Nrf2/HO-1 pathway, so as to improve the antioxidant capacity of the body.

Key words: Ziziphi Spinosae Semen Flavonoids, NF-E2-related factor 2, heme oxygenase-1, perfluorooctane sulfonate, mechanism research