Basic & Clinical Medicine ›› 2021, Vol. 41 ›› Issue (10): 1440-1445.

• Original Articles • Previous Articles     Next Articles

Role of autophagy in hypoxia-reoxygenation-induced injury of rat alveolar epithelial cell type Ⅱ

LIU Xiu-jie1, WANG Xin-yun1, SONG Zheng-yang1, LOU Guo-qiang1, HAO Mao-lin1, QIAN Wei2, WANG Wan-tie1*   

  1. 1. Institute of Ischemia/Reperfusion Injury, Wenzhou Medical University, Wenzhou 325035;
    2. Department of General Medicine, Lin'an District People's Hospital, Hangzhou 311300, China
  • Received:2021-04-30 Revised:2021-07-20 Published:2021-09-29
  • Contact: *wwt@wmu.edu.cn

Abstract: Objective To investigate the role of autophagy in the hypoxia-reoxygenation (H/R) model of rat alveolar epithelial cell type Ⅱ (AEC Ⅱ). Methods Cells were randomly divided into control group (C),hypoxia-reoxygenation group (H/R),solvent group (HD),autophagy agonist group(Rap) and autophagy inhibitor group (3-MA).Pretreated cells with 3-mA 1h before hypoxia. The cell viability was determined with CCK-8, and used RT-PCR, Western blot to detect the expression of autophagy related mRNA transmission and its proteins, and ultrastructure of cell was examined by electron microscopy. Results Compared to the C group, the cell viability decreased, the expression of autophagy related proteins and gene transcription increased, and the cell ultra-structure was significantly damaged (P<0.05). The cell viability in 3-MA group was higher than that of H/R group, the autophagy related proteins and their gene transcription were all decreased (P<0.05), the cell structure was damaged slightly,and autophagy body was not found. Conclusions Hypoxia-reoxygenation can activate autophagy and induce alveolar epithelial cell type Ⅱ damage.

Key words: hypoxia-reoxygenation injury, autophagy, alveolar epithelial cell type Ⅱ

CLC Number: