Abstract: Objective To explore the effect of over-expression of endoplasmic reticulum protein 29 (ERP29) on the invasion and migration of human ovarian cancer OVCAR3 cells, and potentially related mechanisms. Methods The OVCAR3 cell line at logarithmic proliferation stage was divided into over-expression group, empty vector group and control group. The over-expression group was transfected with ERP29 over-expression RNA recombinant eukaryotic vector pcDNA3.1-ERP29 plasmid by liposome transfection method. The empty vector group was transfected with the negative control vector pcDNA3.1 empty plasmid without any treatment. The transfection efficiency was examined by inverted fluorescence microscopy, The expression of ERP29 mRNA after transfection was measured by RT-qPCR. Transwell chamber experiment was used to detect the invasion. The scratch experiment was used to detect the migration. Western blot was used to detect the expression of AKT, p-AKT, mTOR, p-mTOR, 4EBP1, p-4EBP1 protein. Results The plasmid transfection efficiency in over- expression group and empty vector group all exceeded 80%. Compared with the control group and the empty vector group, the relative expression of ERP29 mRNA in the over-expression group was increased (P＜0.05), the number of transmembrane cells ,migration rate and p-AKT/AKT, p-mTOR/mTOR, p-4EBP1/4EBP1 were all decreased(P＜0.05). Conclusions Over-expression of ERP29 can significantly inhibit the invasion and migration of ovarian cancer OVCAR3 cells,the underline mechanism is potentially the inhibition by down-regulating phosphorylation of AKT, mTOR, and 4EBP1.

Key words: ovarian cancer, endoplasmic reticulum protein 29, invasion, migration