Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (9): 1230-1236.

• Original Articles • Previous Articles     Next Articles

hsa-miR-4483 promotes the proliferation and migration of breast cancer cell lines

YANG De-quan1, LI Chang-you2, CUI Jun-wei2, WEI Wei2, WANG Shao-ping1*   

  1. 1. Department of Hepatobiliary Surgery, the Eighth Affiliated Hospital,Sun Yat-sen University,Shenzhen 518000;
    2. Department of Thyroid and Breast Surgery, Peking University Shenzhen Hospital,Shenzhen 518000, China
  • Received:2019-08-26 Revised:2019-12-06 Online:2020-09-05 Published:2020-09-04
  • Contact: *snakewsp@163.com

Abstract: Objective To explore the expression and function of hsa-miR-4483 in breast cancer cells. Methods Using TargetScan to find out how can-miR-4483 act on the c-Myc gene, and RT-qPCR verified the expression of hsa-miR-4483. Transfected MCF-7 and MDA-MB-231 and set up NC-negative controls, Western blot was used to detect the expression of the hsa-miR-4483 gene, MTT assay and Transwell chamber experiment were applied to verify the proliferation and migration of breast cancer cells. Results The expression of hsa-miR-4483 was significantly lower (P<0.001, P<0.01) in MCF-7 and MDA-MB-231(P<0.001, P<0.01) The up-regulation of c-myc in MCF-7 and MDA-MB-231 promoted cell proliferation and migration (P<0.001). Conclusions hsa-miR-4483 can promote the proliferation and migration of breast cancer cell lines, which plays a regulatory role in the development of breast cancer and its prognosis.

Key words: microRNA, breast cancer, proliferation, migration, proliferation curve

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