Abstract: Objective To explore the effect and mechanism of miR-299-3p effects on the proliferation, migration and invasion of renal cell carcinoma cell lines. Methods RT-qPCR was used to detect the expression of miR-299-3p in renal cancer cell lines (786-O, 769-P, ACHN and A498) and human normal renal epithelial cell line(HK-2). MTT assay, wound healing assay, and Transwell assay were used to detect the effects of miR-299-3p on proliferation, migration, and invasion of 786-O cells. Online bioinformatics database was used to predict the target genes of miR-299-3p. Western blot analyzed the expression of E-cadherin,N-cadherin,vimentin and MAP3K12 in 786-O cells. Results miR-299-3p was lower expression in renal cancer cell lines and the lowest expression was found in 786-O cells. Over-expression of miR-299-3p significantly inhibited the proliferation, migration, invasion, tumor xenograft and EMT in 786-O cell and four databases predicted MAP3ZK12 as the common target of miR-299-3p. Dual luciferase reporter gene verified the targeting relationship between miR-299-3p and MAP3K12. miR-299-3p+MAP3K12 effectively reversed the inhibition of miR-299-3p mimic induced cell migration and invasion in 786-O cells. Conclusions The mechanism of miR-299-3p in renal cancer cells may be explained by down-regulated MAP3K12 to affect cell proliferation, migration, invasion and EMT pathway.

Key words: renal carcinoma, 786-O cell, miR-299-3p, MAP3K12, epithelial mesenchymal transition (EMT)