Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (8): 1076-1082.

• Original Articles • Previous Articles     Next Articles

TXNIP reduces pro-proliferation effect of GLP-1 on mouse islet β cells

ZHANG Wen-ting, WANG Jin, YUE Ji-ping, JIN Wen-wen, ZHANG Zhi-nan, JIAO Xiang-ying*   

  1. Department of Physiology, Shanxi Medical University, Taiyuan 030001, China
  • Received:2019-08-26 Revised:2019-12-03 Online:2020-08-05 Published:2020-07-29
  • Contact: *jiaoxyty@163.com

Abstract: Objective To observe the role of thioredoxin interacting protein (TXNIP) in the proliferation of islet β cells promoted by Glucagon-like peptide 1 (GLP-1). Methods The mice were divided into db/m group and db/db group; TXNIP over-expression (Ad-TXNIP-GFP) MIN6 cells were constructed by stably transfected with lentivirus, which were divided into control group, Ad-GFP group and Ad-TXNIP-GFP group. Western blot was used to detect the expression of TXNIP and proliferating cell nuclear antigen (PCNA) in pancreatic tissues of db/m and db/db mice. The morphology of the islets, the number of β cells, GLP-1R and Ki67 were observed by HE and immunohistochemistry method. The expression of GLP-1 in serum was detected by ELISA. The level of GLP-1R protein in pancreatic tissue was detected by immunohistochemistry. Results Islet structure was destructed, the number of β cells and PCNA expression decreased in pancreatic tissue of db/db mice (P<0.05). The level of GLP-1 in serum reduced (P<0.05), and the level of GLP-1 receptor in pancreatic tissue and islet was reduced (P<0.05). The expression of TXNIP in pancreatic tissues of db/db mice increased (P<0.05). The TXNIP overexpression cell model was successfully constructed (P<0.05), and the level of GLP-1 receptor decreased in islet β cells (P<0.05). The overexpression of TXNIP reduced the proliferative effect of islet β cells to GLP-1 (P<0.05). Conclusions TXNIP acts on the GLP-1 receptor to reduce the proliferation of islet β cells promoted by GLP-1.

Key words: thioredoxin interacting protein, GLP-1, GLP-1 receptor, cell proliferation

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