miR-29b inhibits LPS-induced apoptosis of alveolar epithelial cell line A549 through regulating Bax gene

Abstract

Abstract: Objective To investigate the effect of miR-29b on LPS-induced apoptosis of alveolar epithelial cell line A549 and its mechanism. Methods A549 cells were divided into control group, LPS group (treated with 10 mg/L LPS), LPS+miR-NC group (treated with LPS after transfection of miR-29b mimics negative control), LPS+miR-29b group(treated with LPS after transfection of miR-29b mimics), the expression level of miR-29b in each group was detected by RT-qPCR, cell viability was detected by MTT assay, and apoptosis rate was detected by flow cytometry.The expressions of Bcl-2, Bax and cleaved caspase-3 protein were detected by Western blot, and the dual luciferase reporter gene assay was used to detect the targeting relationship between Bax and miR-29b. Results Compared with the control group, the expression levels of miR-29b and Bcl-2 proteins and cell viability in the LPS group, LPS+miR-NC group and LPS+miR-29b group were significantly decreased, while the apoptosis rate was decreased. The expression of Bax and cleaved caspase-3 proteins significantly increased (P＜0.05). Compared with LPS group, the expression of miR-29b and Bcl-2 proteins and cell survival rate in LPS+miR-29b cells were significantly higher, while the apoptotic rate, the expression of Bax and cleaved caspase-3 protein were all significantly lower(P＜0.05). Dual luciferase reporter assays confirmed that Bax could be a potential target gene for miR-29b. Conclusions miR-29b can inhibit the apoptosis of alveolar epithelial cells A549 induced by LPS, and its mechanism may be related to the targeted regulation of Bax expression.

Key words: alveolar epithelial cells, miR-29b, LPS, apoptosis, Bax

CLC Number:

R966

PAN Shu, WEN Dan-ning, LI Hua-dong. miR-29b inhibits LPS-induced apoptosis of alveolar epithelial cell line A549 through regulating Bax gene[J]. Basic & Clinical Medicine, 2020, 40(6): 797-802.

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