RNA localization in cytoplasm and nucleus of human embryonic stem cells

Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (6): 765-770.

• Original Articles • Previous Articles Next Articles

RNA localization in cytoplasm and nucleus of human embryonic stem cells

ZHOU Fan-qi¹, FENG Wen-xin², TAN Pu-wen³, MA Yan-ni¹, WANG Dong³, YU Jia^1*

1. State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005;
2. Henan Geology Vocational College, Zhengzhou 450000;
3. Department of Bioinformatics, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510000, China

Received:2020-03-08 Revised:2020-04-19 Online:2020-06-05 Published:2020-05-29
Contact: *j-yu@ibms.pumc.edu.cn

Abstract

Abstract: Objective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used to verify the localization of these RNAs. Results More RNA types were found in the cytoplasm than nucleus, as well as specifically locational RNAs (2 952 in the cytoplasm and 634 in the nucleus, respectively). Conclusions The localization map of RNA in cytoplasm and nucleus of hESCs is described,and the localization of many RNAs is verified.

Key words: human embryonic stem cells (hESCs), RNA localization

CLC Number:

Q522

ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia. RNA localization in cytoplasm and nucleus of human embryonic stem cells[J]. Basic & Clinical Medicine, 2020, 40(6): 765-770.

References

[1] Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts[J]. Science, 1998, 282: 1145-1147.
[2] Reubinoff BE, Pera MF, Fong C, et al. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro[J]. Nat Biotechnol, 2000, 18: 399-404.
[3] Zarei-Kheirabadi M, Sadrosadat H, Mohammadshirazi A, et al. Human embryonic stem cell-derived neural stem cells encapsulated in hyaluronic acid promotes regenera-tion in a contusion spinal cord injured rat[J]. Int J Biol Macromol, 2020, 148: 1118-1129.
[4] Eguizabal C, Aran B, Chuva De Sousa Lopes SM, et al. Two decades of embryonic stem cells: a historical overview[J]. Hum Reprod Open, 2019.doi:10.1093/hropen/hoy024.
[5] Bertrand E, Chartrand P, Schaefer M, et al. Localization of ASH1 mRNA particles in living yeast[J]. Mol Cell, 1998, 2: 437-445.
[6] Chartrand P, Meng X, Singer RH, et al. Structural elements required for the localization of ASH1 mRNA and of a green fluorescent protein reporter particle in vivo[J]. Curr Biol, 1999, 9: 333-338.
[7] Ghosh S, Marchand V, Gáspár I, et al. Control of RNP motility and localization by a splicing-dependent structure in oskar mRNA[J]. Nat Struct Mol Biol, 2012, 19: 441-449.
[8] Grünwald D, Singer RH. In vivo imaging of labelled endogenous β-actin mRNA during nucleocytoplasmic transport[J]. Nature, 2010, 467: 604-607.
[9] Weil TT, Forrest KM, Gavis ER. Localization of bicoid mRNA in late oocytes is maintained by continual active transport[J]. Dev Cell, 2006, 11: 251-262.
[10] Lécuyer E, Yoshida H, Parthasarathy N, et al. Global Analysis of mRNA localization reveals a prominent role in organizing cellular architecture and function[J]. Cell, 2007, 131: 174-187.
[11] Martin KC, Ephrussi A. mRNA localization: gene expression in the spatial dimension[J]. Cell, 2009, 136: 719-730.
[12] Buxbaum AR, Haimovich G, Singer RH. In the right place at the right time: visualizing and understanding mRNA localization[J]. Nat Rev Mol Cell Biol, 2015, 16: 95-109.
[13] Bahar Halpern K, Caspi I, Lemze D, et al. Nuclear retention of mRNA in mammalian tissues[J]. Cell Rep, 2015, 13: 2653-2662.
[14] Fazal FM, Han S, Parker KR, et al. Atlas of subcellular RNA localization revealed by APEX-Seq[J]. Cell, 2019, 178: 473-490.
[15] Palazzo AF, Lee ES. Sequence determinants for nuclear retention and cytoplasmic export of mRNAs and lncRNAs[J]. Front Genet, 2018, 9:440.

RNA localization in cytoplasm and nucleus of human embryonic stem cells

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	LIU Man, REN Yue, GAO Yu-feng, WANG Fang, YU Jia. Translational regulation of human embryonic kidney cell line HEK293 [J]. Basic & Clinical Medicine, 2021, 41(5): 630-635.
[2]	. Validation of intergenic long non-coding RNA: T-UCRs for their transcriptional activities [J]. Basic & Clinical Medicine, 2015, 35(5): 632-636.
[3]	. The analysis of coding genes expression during the differentiation of neural stem cells [J]. Basic & Clinical Medicine, 2014, 34(6): 734-739.
[4]	. Suprachiasmatic nucleus lesion induced abnormal rhythm of circadian inflammatory pain [J]. Basic & Clinical Medicine, 2014, 34(6): 787-791.
[5]	. The natural antisense transcript, Lmo4as, negatively regulated the same locus cortical coding gene Lmo4 [J]. Basic & Clinical Medicine, 2014, 34(6): 819-823.
[6]	. Screening and identification of long noncoding RNAs in PCBP2 gene locus [J]. Basic & Clinical Medicine, 2013, 33(6): 694-698.
[7]	Da-quan LIU; Dong-hua LI; Hong-bin LIU. MiR-141 increases proliferation and migration of human vascular endothelial cells [J]. Basic & Clinical Medicine, 2010, 30(8): 801-806.