Comparison of liquid-phase chip and real-time fluorescent quantitative PCR for the detection of infectious diarrhoeal pathogens in children

Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (4): 523-527.

• Original Articles • Previous Articles Next Articles

Comparison of liquid-phase chip and real-time fluorescent quantitative PCR for the detection of infectious diarrhoeal pathogens in children

HAN Cong-hui¹, CAO Guang-jin², ZHANG Fu-zhen¹, HU Liang-shan³, CAO Dong-lin^3*, LI Ling^1*

1. Biosafety Level 3 Laboratory, School of Public Health, Southern Medical University, Guangzhou 510515;
2. Panyu District Center for Disease Control and Prevention, Guangzhou 511400;
3. Department of Laboratory Medicine, Guangdong Second Provincial General Hospital, Guangzhou 510317, China

Received:2019-11-12 Revised:2020-01-15 Online:2020-04-05 Published:2020-04-06
Contact: *liling@smu.edu.cn; caodl@126.com

Abstract

Abstract: Objective To investigate the efficiency of liquid-phase chip on the detection of infectious diarrhea pathogens in children, and provide reference for rapid clinical diagnosis of infectious diarrhea diseases. Methods Stool samples of 96 infants and children with diarrhea were collected and detected by xTAG®Gastrointestinal Pathogen Panel (GPP), and the results were compared with the real-time PCR method. Results Total of 7 pathogens were detected by liquid-phase chip technology, with a positive detection rate of 65.63%(63/96), and the Salmonella detection rate was highest as 50%(48/96). The positive rate of single pathogen infection was 39.58%(38/96), and the positive rate of mixed infection was 26.04%(25/96). Overall, compared with the results of real-time PCR, the sensitivity and specificity of the liquid-phase chip in the detection of diarrhea pathogens were 100% and 71.74%, respectively, and negative predictive value and positive predictive value were 100.00% and 79.37%, respectively(P＜0.05). Conclusions Compared with real-time PCR, liquid-phase chip is a character-ized by high throughput, rapid and reliable so support laboratory diagnosis of contagious diarrhea

Key words: liquid-phase chip, real-time PCR, infectious diarrhea diseases, pathogen detection

CLC Number:

R331

HAN Cong-hui, CAO Guang-jin, ZHANG Fu-zhen, HU Liang-shan, CAO Dong-lin, LI Ling. Comparison of liquid-phase chip and real-time fluorescent quantitative PCR for the detection of infectious diarrhoeal pathogens in children[J]. Basic & Clinical Medicine, 2020, 40(4): 523-527.

References

[1]梁丹, 车荣飞, 石倩萍, 等. 感染性腹泻疾病负担及其病原谱变化的研究进展[J].中国公共卫生, 2019, 35: 467-470.
[2]GBD 2016 Disease and Injury Incidence and Prevalence Collaborators. Global, regional, and national incidence, prevalence, and years lived with disability for 328 diseases and injuries for 195 countries, 1990-2016: a systematic analysis for the Global Burden of Disease Study 2016[J]. Lancet, 2017, 390: 121-159.
[3]GBD 2017 Causes of Death Collaborators. Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980-2017: a systematic analysis for the Global Burden of Disease Study, 2017[J]. Lancet, 2018, 392: 1736-1788.
[4]Chrisra L, Fischer W, Li Liu, et al. Global burden of childhood pneumonia and dierrbea[J]. Lancet, 2013, 381: 1405-1416.
[5]Li LL, Liu N, Humphries EM, et al. Aetiology of diarrhoeal disease and evaluation of viral-bacterial coinfection in children under 5 years old in China: a matched case-control study[J]. Clin Microbiol Infect, 2016, 22: 9-16.
[6]闫俊平, 曹东林. 液相芯片技术在传染病快速诊断中的应用[J]. 现代医院, 2017, 17: 1782-1785.
[7]曹广进. 利用液相芯片技术检测儿童急性呼吸道感染及感染性腹泻病原体的研究[D]. 广州: 南方医科大学, 2019: 34-79.
[8]Kotloff KL, Platts-Mills JA, Nasrin D, et al. Global burden of diarrheal diseases among children in developing countries:Incidence, etiology, and insights from new molecular diagnostic techniques[J]. Vaccine, 2017, 35: 6783-6789.
[9]Tickell KD, Brander RL, Atlas HE, et al. Identification and management of Shigella infection in children with diarrhoea: a systematic review and meta-analysis[J]. Lancet Glob Health, 2017, 5: 1235-1248.
[10]黄恩妙, 王翠玲, 王曼, 等. 2015-2016年中山市感染性腹泻病原学监测结果分析[J].现代预防医学, 2017, 44: 3040-3047.
[11]Casañ C, Ocete MD, Medina R, et al. Evaluation of the Luminex xTAG®GPP (Gastrointestinal Pathogen Panel) in the diagnosis of diseases with acute diarrhoea[J]. Enferm Infecc Microbiol Clin, 2017, 35: 574-577.
[12]Porte L, Varela C, Haecker T, et al. Impact of changing from staining to culture techniques on detection rates of Campylobacter spp. In routine stool samples in Chile[J]. BMC Infect Dis, 2016, 16: 196-202.
[13]Deng J, Luo X, Wang R, et al. A comparison of Luminex xTAG®Gastrointestinal Pathogen Panel (xTAG GPP) and routine tests for the detection of enteropathogens circulating in Southern China[J]. Diagn Microbiol Infect Dis, 2015, 83: 325-330.
[14]林丽娟, 彭雅冰, 罗欣, 等. 悬浮芯片法调查广州腹泻病原体分布及临床特征分析[J]. 实用医学杂志, 2016, 32: 3724-3727.

Comparison of liquid-phase chip and real-time fluorescent quantitative PCR for the detection of infectious diarrhoeal pathogens in children

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	. Correlation of minimal residual disease and multidrug resistance genes expression in acute myeloid leukemia [J]. Basic & Clinical Medicine, 2017, 37(1): 62-66.
[2]	. Expression and significance of ADAM17 in papillary carcinoma of thyroid [J]. Basic & Clinical Medicine, 2016, 36(3): 393-395.
[3]	. The expression of miRNA-143 during adipogenic differentiation of MSCs [J]. Basic & Clinical Medicine, 2012, 32(1): 12-15.
[4]	Bing WANG; Hong-liang LI; Wen-ying YANG; Jian-zhong XIAO; Rui-qin DU; Xiu-ping BAI; Lin PAN. NAC on the improvement of insulin resistance induced by FFA in rats [J]. Basic & Clinical Medicine, 2008, 28(6): 549-552.