Estrogen promotes proliferation and invasion of astrocyte cell line U-87

Abstract

Abstract: Objective To explore the relationships between the estrogen and astrocyte cell proliferation as well as invasion. Methods Astrocyte cell line U-87 was cultured in vitro and treated with 0, 10^－12, 10^－10, 10^－8 and 10^－6 mol/L estrogen. The gene and protein expressions were analyzed by qPCR, Western blot, ELISA; and the cell proliferation and invasion was analyzed by CCK-8 kits and transwell experiments. Results Estrogen significantly inhibited IL-1, TNF-α, IL-6 expression and promoted the expression of NGF and PGE2 (P＜0.05). Furthermore, estrogen inhibited aquaporin 2(AQP2) expression (P＜0.05). The proliferation and invasion experiments showed that estrogen promoted the U-87 cell proliferation and invasion (P＜0.05). Conclusions Estrogen down-regulates the expression of inflammatory factors, inhibites the expression of AQP2, promotes the proliferation and invasion of U-87 cells.

Key words: estrogen, astrocyte, aquaporin 2, proliferation

CLC Number:

LIU Yan-min, FEI Yi-lin, LI Xing-miao, WANG Ning, CHEN Mo, JIANG Xiu-xiu. Estrogen promotes proliferation and invasion of astrocyte cell line U-87[J]. Basic & Clinical Medicine, 2020, 40(3): 305-309.

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