Recombinant human TNC induces EMT, migration and invasion of pancreatic cancer cell line PANC1

Abstract

Abstract: Objective To construct human TNC expression plasmid for the investigation of the role of TNC in pancreatic cancer metastasis by using pancreatic cancer cell strain with stable over-expression of TNC. Methods TNC expression plasmid was constructed by unique primer design method, and then pancreatic cancer cell strain PANC1 with stable TNC expression were obtained by transfection and G418 screening. RT-qPCR and Western blot examined the expression of TNC. Transwell and Western blot were used to detecte the effects of TNC on cell migration, invasion and EMT-related molecules. Results TNC plasmid was successfully constructed. TNC promoted cell migration and invasion(P＜0.05), decreased epithelial marker E-cadherin expression and upregulated mesenchymal marker N-cadherin expression (P＜0.05). Conclusions The TNC expression plasmid and TNC stable expression pancreatic cancer cell strain may be used to explore the biological function of TNC and the pathogenesis of pancreatic cancer.

Key words: TNC, pancreatic cancer, migration, invasion, EMT

CLC Number:

R735.9

CAI Jun, SHEN Wen-yuan, WANG Hui, TAN Cheng, ZHAO Xiu-mei, SHEN Xiao-hong. Recombinant human TNC induces EMT, migration and invasion of pancreatic cancer cell line PANC1[J]. Basic & Clinical Medicine, 2020, 40(2): 167-172.

References

[1]Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019 [J]. CA Cancer J Clin, 2019, 69: 7-34.
[2]Esposito I, Penzel R, Chaib-Harrireche M, et al. Tenascin C and annexin II expression in the process of pancreatic carcinogenesis [J]. J Pathol, 2006, 208: 673-685.
[3]Cai J, Lu W, Du S, et al. Tenascin-C modulates cell cycle progression to enhance tumour cell proliferation through AKT/FOXO1 signalling in pancreatic cancer [J]. J Cancer, 2018, 9: 4449-4462.
[4]Whittle MC, Hingorani SR. Fibroblasts in pancreatic ductal adenocarcinoma: biological mechanisms and therapeutic targets [J]. Gastroenterology, 2019, 156: 2085-2096.
[5]Sun Z, Schwenzer A, Rupp T, et al. Tenascin-C promotes tumor cell migration and metastasis through integrin alpha9beta1-mediated YAP inhibition [J]. Cancer Res, 2018, 78: 950-961.
[6]Murakami T, Kikuchi H, Ishimatsu H, et al. Tenascin C in colorectal cancer stroma is a predictive marker for liver metastasis and is a potent target of miR-198 as identified by microRNA analysis [J]. Br J Cancer, 2017, 117: 1360-1370.
[7]Kropac AC, Eshwar AK, Stephan R, et al. New insights on the role of the pLMST6 plasmid in listeria monocytogenes biocide tolerance and virulence [J]. Front Microbiol, 2019, 10. doi: 10.3389/fmicb.2019.01538.
[8]Zhou P, Li B, Liu F, et al. The epithelial to mesen-chymal transition (EMT) and cancer stem cells: implication for treatment resistance in pancreatic cancer [J]. Mol Cancer, 2017, 16. doi: 10.1186/s12943-017-0624-9.
[9]Thomson TM, Balcells C, Cascante M. Metabolic plas-ticity and epithelial-mesenchymal transition [J]. J Clin Med, 2019, 8. doi: 10.3390/jcm8070967.
[10]Takahashi Y, Sawada G, Kurashige J, et al. Tumor-derived tenascin-C promotes the epithelial-mesenchymal transition in colorectal cancer cells [J]. Anticancer Res, 2013, 33: 1927-1934.
[11]Yang Z, Zhang C, Qi W, et al. Tenascin-C as a pro-gnostic determinant of colorectal cancer through induction of epithelial-to-mesenchymal transition and prolifera-tion [J]. Exp Mol Pathol, 2018, 105: 216-222.
[12]Yoshida T, Akatsuka T, Imanaka-Yoshida K. Tenascin-C and integrins in cancer [J]. Cell Adh Migr, 2015, 9: 96-104.

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