Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (11): 1479-1483.

• Original Articles • Previous Articles     Next Articles

Autophagy in CaSR-mediated migration of non-small-cell carcinoma cell line A549

LIU Jia-long, LI Chun-lin, LI Guang-wei*   

  1. Department of Pathophysiology, School of Basic Medicine, Qiqihar Medical University, Qiqihar 161006, China
  • Received:2020-03-22 Revised:2020-06-29 Online:2020-11-05 Published:2020-10-30
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Abstract: Objective To investigate the role of autophagy in hypoxia-activated CaSR(calcium-sensing receptor,CaSR) in mediating migration of A549 cells. Methods A549 cells in the logarithmic growth phase were randomly divided into control group(N), hypoxia group (H), hypoxia agonist group (H+Gd), hypoxia inhibitor group (H+NPS), and autophagy pathway inhibitor group (H+Gd+3-MA).Western blot was used to detect the expression of CaSR and autophagy protein (Beclin-1) in the cells; Cell scratch experiment was used to detect migration of A549 cells; Transwell assay was applied to evaluate the invasion of A549 cells. Results Hypoxia resulted in increased CaSR expression in A549 cells (P<0.05), a decreased expression of autophagic effector protein Beclin-1(P<0.05), increased the percentage of cell healing per unit time (P<0.05), and the number of cell invasion-permeable membranes (P<0.05).GdCl3 strengthened the effect of hypoxia, while NPS2390 did weaken the effect of hypoxia. On the basis of hypoxia and GdCl3 conditions, 3-MA further increased the expression of metastasis related indicators of A549 cells, but not the autophagy related indicators. Conclusions CaSR activation promotes migration and invasion of A549 cells by down-regulating autophagy.

Key words: A549 cell, hypoxia, autophagy, migration and invasion

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