Basic & Clinical Medicine ›› 2020, Vol. 40 ›› Issue (11): 1468-1473.

• Original Articles • Previous Articles     Next Articles

Differentiation of human urine-derived iPSCs into hair follicle stem cells

ZHOU Hua-teng*, LI Chang-ming, WANG Li-xiang, HU Jin-tao, DU Wei-bin, ZHANG He-lou, REN Wei-fan   

  1. Department of Orthopedics, Affiliated Jiangnan Hospital of Zhejiang Chinese Medical University, Hangzhou 311201, China
  • Received:2020-05-17 Revised:2020-07-27 Online:2020-11-05 Published:2020-10-30
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Abstract: Objective To differentiate urine derived pluripotent stem cells (iPSCs) into hair follicle stem cells (HFSCs) to construct a skin substitutes. Methods Human urinary cells isolated from a healthy donor were re-programmed into iPSCs. iPSCs were then induced into iPS-HFSCs. Immunocytochemistry and RT-qPCR were used to examine the characteristics of the induced iPS-HFSCs. Results The iPSCs induced from urine cells have characteristics as embryonic stem cells. iPSCs derived cells displayed the morphology, genes expression and phenotypes similar to iPS-HFSCs. Conclusions The urine cells are successfully reprogramed into iPSCs by four transcription factors, and iPSCs derived cells show characteristics similar to HFSCs.

Key words: EGF, induced pluripotent stem cells, hair follicle stem cells

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