Dehydrocostuslactone inhibits proliferation and promotes apoptosis of human hepatocellular carcinoma cell line HepG2

Abstract

Abstract: Objective To investigate the effects of dehydrocostuslactone treatment on the proliferation and apoptosis of human HepG2 cells and potential mechanisms. Methods HepG2 cells were divided into 6 groups incubated with different concentration of Dehydrocostuslactone. The cell proliferation was evaluated by MTT assays, and Hoechst 33258 fluorescence staining was conducted to observe apoptosis of cells subsequently.The expression of p-IκBα, IκBα, nuclear p65 and total p65 proteins in HepG2 cells were measured using Western blot. Results Compared with the control group, dehydrocostuslacton,in a dose dependent manner, significantly inhibited the proliferation of HepG2 cells with impressive profile of apoptosis (P＜0.05 or P＜0.01). Dehydrocostuslactone, at concentrations of 10, 20 and 30 μmol/L, resulted in a significantly decrease of p-IκBα and nuclear p65 expression (P＜0.05 or P＜0.01) and an increased IκBα expression (P＜0.05 or P＜0.01), whereas no marked effect on total p65 expression was observed. Conclusions Dehydrocostuslactone inhibits proliferation and promotes apoptosis of HepG2 cells, the mechanism may be explained by the down-regulation of NF-κB pathway in HepG2 cell.

Key words: dehydrocostuslactone, HepG2 cells, NF-κB pathway, cell proliferation, apoptosis

CLC Number:

R735.7

MIAO Jia-wei, CHEN Jie, DENG Xue-song, XIONG Shu, LI Guo-li, MA Qiang. Dehydrocostuslactone inhibits proliferation and promotes apoptosis of human hepatocellular carcinoma cell line HepG2[J]. Basic & Clinical Medicine, 2020, 40(10): 1369-1373.

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