Effect of abdominal paracentesis drainage on TLR4 expression in liver of rats with early severe acute pancreatitis

Abstract

Abstract: Objective To investigate the effect of abdominal paracentesis drainage (APD) on TLR4 expression in the liver of rats with early severe acute pancreatitis (SAP) and its significance. Methods The rats were randomly divided into sham operation group (sham group), SAP group(SAP model was established by retrograde injection of 5% sodium trasulfide cholate into the pancreatic bile duct) and SAP+APD group(drainage tube was placed and fixed on the basis of SAP). HE staining, automatic biochemical analyzer, ELISA, real-time PCR and Western blot were used to detect relevant indexes. Results Compared with sham group, the pathological injury of liver and pancreas were significantly worse and the pathological scores were significantly increased in the SAP group (P＜0.05), the activityies of serum amylase and lipase, serum indicators related to liver function, and serum levels of TNF-α, IL-1β were also significantly increased (P＜0.05); The mRNA and protein expressions of TLR4, the protein expression of NF-κB p65, TNF-α, IL-1β , and the phosphorylation levels of NF-κB p65 in liver tissues in the SAP group were significantly up-regulated than those in the sham group (P＜0.05); After APD intervention,the above indicators were significantly alleviated (P＜0.05). Conclusions Implementation of APD can significantly decrease the expression of TLR4 in liver tissue of rats with early SAP, inhibit the activation of downstream NF-κB signaling pathway, thus relieve the degree of liver injury.

Key words: severe acute pancreatitis, abdominal paracentesis drainage, liver injury, Toll-like receptor 4, high mobility group box-1 protein

CLC Number:

R657.5⁺1

CHEN Zhen-yu, HUANG Qi-lin, SUN Hong-yu, LIU Li-ye, WEN Yi. Effect of abdominal paracentesis drainage on TLR4 expression in liver of rats with early severe acute pancreatitis[J]. Basic & Clinical Medicine, 2020, 40(10): 1342-1347.

References

[1] Forsmark CE, Vege SS, Wilcox CM. Acute pancreatitis[J]. N Engl J Med, 2016, 375: 1972-1981.
[2] 徐彦哲, 丁佑铭, 汪斌, 等. 急性胰腺炎肝损伤的研究进展[J]. 中华肝胆外科杂志, 2015, 2:284-288.
[3] Liu WH, Ren LN, Chen T, et al. Abdominal paracentesis drainage ahead of percutaneous catheter drainage benefits patients attacked by acute pancreatitis with fluid collections: a retrospective clinical cohort study[J]. Crit Care Med, 2015, 43: 109-119.
[4] Schmidt J, Rattner DW, Lewandrowski K, et al. A better model of acute pancreatitis for evaluating therapy[J]. Ann Surg, 1992, 215: 44-56.
[5] Camargo CA, Madden JF, Gao WS, et al. Interleukin-6 protects liver against warm ischemia/reperfusion injury and promotes hepatocyte proliferation in the rodent[J]. Hepatology, 1997, 26: 1513-1520.
[6] Sha HC, Ma QY, Jha RK. Trypsin is the culprit of multiple organ injury with severe acute pancreatitis[J]. Med Hypotheses, 2009, 72: 180-182.
[7] Samanta J, Rana A, Dhaka N, et al. Ascites in acute pancreatitis: not a silent bystander[J]. Pancreatology, 2019, 19: 646-652.
[8] Liu L, Yan H, Liu W, et al. Abdominal paracentesis drainage does not increase infection in severe acute pancreatitis: a prospective study[J]. J Clin Gastroenterol, 2015, 49:757-763.
[9] Garcia MM, Goicoechea C, Molina-Alvarez M, et al. Toll-like receptor 4: a promising crossroads in the diagno-sis and treatment of several pathologies[J].Eur J Pharmacol, 2020, 874: 172975.doi: 10.1016/j.ejphar.2020.172975.
[10] Wang Q, Zhang X, Lei SH, et al. RNA sequence analysis reveals pathways and candidate genes associated with liver injury in a rat pancreatitis model[J]. Pancreatology, 2018, 18: 753-763.
[11] Zhang L, Wu HS, Chen Y, et al. Role of nitric oxide in Toll-like receptor 2 and 4 mRNA expression in liver of acute hemorrhagic necrotizing pancreatitis rats[J]. World J Gastroenterol, 2006, 12: 485-488.
[12] Peng Y, Sigua CA, Rideout D, et al. Deletion of toll-like receptor-4 downregulates protein kinase C-zeta and attenuates liver injury in experimental pancreatitis[J]. Surgery, 2008, 143: 679-685.
[13] Erlandsson Harris H, Andersson U. Mini-review: the nuclear protein HMGB1 as a proinflammatory mediator[J]. Eur J Immunol, 2004, 34: 1503-1512.
[14] Wen Y, Sun HY, Tan Z, et al. Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxida-tive stress[J]. World J Gastroenterol, 2020, 26: 35-54.