Pantoprazole induces bradyarrhythmia in mice with hypokalemia

Abstract

Abstract: Objective To investigate the mechanism of arrhythmia induced by pantoprazole (PPZ) in mice with hypokalemia. Methods Mice were randomly divided into control group, PPZ group [20 mg/(kg·d), intraperitoneal injection for 5 weeks], furosemide (FS) group and FS+PPZ group. After 5 weeks, the ECG parameters (heart rate, PR interval, QRS interval, QTc interval) and spontaneous arrhythmia were monitored. The changes of the mRNA and protein expression of hyperpolarization-activated and cyclic nucleotide-cation channels 2 and 4 (HCN2 and HCN4), cardiac voltage-gated sodium channel alpha subunit (SCN5A), L-type calcium channel alpha subunit (CACNA1C) and T-type calcium channel alpha subunit (CACNA1G) were analysed by real-time fluorescence quantitative PCR and Western blot technology. Results Compared with control group, the HR was significantly decreased and PR interval was prolonged in PPZ group (P＜0.01, P＜0.05). Compared with FS group,the HR and PR interval in FS+PPZ group were further decreased and prolonged (P＜0.01, P＜0.05), and 4 mice developed sinus arrest (SA) (P＜0.05). The expressions of HCN4 mRNA and protein in PPZ group were lower than those in control group (P＜0.01), respectively, and the expression in FS+PPZ group was lower than those in FS group (P＜0.01). The expression of HCN2 mRNA in PPZ group was lower than that in control group(P＜0.05), which was further decreased in FS+PPZ group compared with FS group (P＜0.01). Conclusions It is found that pantoprazole may lead to bradyarrhythmia in mice under hypokalemia, which may be related to abnormal expression of HCN2 and HCN4.

Key words: pantoprazole, hypokalemia, arrhythmia, mechanism

CLC Number:

R541.7

LI Shuai, ZHU Wen-gen, YAN Xia, LAI Wei, YU Jian-hua, WAN Rong, HONG Kui. Pantoprazole induces bradyarrhythmia in mice with hypokalemia[J]. Basic & Clinical Medicine, 2020, 40(1): 73-77.

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