Basic & Clinical Medicine ›› 2019, Vol. 39 ›› Issue (7): 961-966.

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Tectoridin and aucubin inhibit LPS-induced inflammatory response of human adipose mesenchymal stem cells

  

  • Received:2019-04-19 Revised:2019-05-22 Online:2019-07-05 Published:2019-07-02

Abstract: Objective To study the effects of tectoridin and aucubin on the immune regulation of human adipose mesenchymal stem cells (hAMSCs) induced by lipopolysaccharide (LPS).Methods CCK-8 cell proliferation test and cell cycle test were used to screen the appropriate concentration of drugs; LPS concentration was screened to establish an inflammatory response model; ELISA and RT-q PCR were used to detect the effects of both on the secretion of pro-inflammatory and anti-inflammatory factors by hAMSCs after LPS induction. Results 50μg/mL tectoridin and 100μg/mL aucubin could significantly promote the proliferation of normal hAMSCs (P < 0.05 ,P<0.01). Both of them promoted the proliferation of hAMSCs induced by LPS (P<0.001), and inhibited the secretion of IL-6,TNF-α,IFN-γ (P<0.05 ,P<0.01) .Conclusions Tectoridin and aucubin could significantly inhibit LPS-induced hAMSCs inflammatory response.

Key words: human adipose mesenchymal stem cells, inflammatory cytokines, immune regulation, psoriasis