Abstract: Objective To investigate how PRMT1 regulates c-myc in breast cancer cells. Methods The expression of c-myc was detected in protein and mRNA levles in overexpressing C/EBPα and knocking down PRMT1 of MDA-MB-231 cells. ChIP-qPCR and EMSA were performed to detect the binding ability of C/EBPα to the promoter of c-myc. The promoter of c-myc activity regulated by PRMT1 and C/EBPα was detected by dual-luciferase report system. Results The expression of c-myc declined dramatically after overexpressing C/EBPα and knocking down PRMT1. The promoter of c-myc activity repressed by C/EBPα was weakened obviously after the methylation sites of C/EBPα at R35F, R156F and R165F were mutated. The regulation of c-myc by PRMT1 has a little effect after the methylation sites of C/EBPα at R35K, R156K and R165K were mutated. Conclusions PRMT1 regulates the transcription of c-myc by methylation of C/EBPα.

Key words: Key words：PRMT1, C/EBPα, c-Myc, Arginine methylation