Abstract: Feeder cells have been widely used as an assistant method for the in vitro culture of mammal cells.It can simulate the condition of in vivo culture to a certain extent by the secretion of growth factors and the physical support. According to different culture purposes,we may choose specific feeder cells and dealing methods or modify feeder cells through gene editing to obtain the best culture results.

Key words: Feeder cells, Gene editing, Stem cell and iPS