Basic & Clinical Medicine ›› 2019, Vol. 39 ›› Issue (1): 76-80.
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wang can1,
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Abstract: Objective To investigate the effect of BMP4 on glioblastoma cell invasion and its mechanism. Methods In human malignant glioma cells U251, myc, myc-BMP4, siCtrl, siBMP4 was transfected into cells, and changes on the invasive ability of cells with high and low BMP4 expression were detected by Scratch wound healing assay, In mechanism study, RT-PCR and western blot assay were used to detect the expression of E-cadherin and Snail1 in four groups. Results Wound healing results showed that high BMP4 expression in U251 cells decreased cell invasion(P<0.01), and low BMP4 expression increased cell invasion; RT-EPCR and Western blot assay results showed that high BMP4 expression significantly increase E-cadherin expression(P<0.01), low BMP4 expression significantly decrease E-cadherin expression(P<0.01), when BMP4 expression was high, Snail1 protein expression was decreased(P<0.01). Conclusion: BMP4 can regulate GBM cell invasion through negative regulation of Snail1 signaling pathway.
Key words: invasion, BMP4, snail1
wang can. Over-expression of bone morphogenetic protein 4 regulates invasion of human glioblastoma cell line U251[J]. Basic & Clinical Medicine, 2019, 39(1): 76-80.
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http://journal11.magtechjournal.com/Jwk_jcyxylc/EN/Y2019/V39/I1/76