Abstract: Objective To investigate the expression of Neat1 in the differentiation of P19 cells induced by all-trans-retinoic acid and explore the effect of histone modification on its expression. Methods The differentiation of P19 cells were induced with the α-MEM culture media containing 0.5μmol/L all-trans-retinoic(atRA) acid and the expression Mash1 which is the neural differentiation maker gene was measured. The expression of Neat1 was measured with RT-qPCR. The cells were treated with TSA, NAM or AdOx respectively to investigate the effect of the histone modifier inhibitor on the expression of Neat1. Results The model of differentiation of P19 cells induced by atRA was constructed successfully. Mash1 was upregulated significantly in the process of P19 cells differentiation.Neat1 was upregulated significantly with the induction of P19 cells treated with atRA(P<0.01). The TSA but not the NAM or AdOx could induce the expression of the Neat1.Conclusions The expression of Neat1 is upregulated significantly in the process of P19 differentiation induced by atRA and the the high level of histone acetylation maintained by TSA could induce the expression of Neat1.

Key words: Keywords: Neat1, Neural differentiation, TSA