Abstract: Objective To investigate the post-transcriptional regulation mechanism of IGF2, which is an important tumor regulator, by SIRT6. Methods SIRT6 was overexpressed in 293T cells and IP was used to enrich SIRT6 and mass spectrometry（MS）was used to detect the protein that interacted with SIRT6.Western blot was used to validate the interacted protein and its acetylation factor 2 mRNA-binding protein 1(IMP1). SIRT6 did not change the acetylation modification status of IMP1 , but the phosphorylation modification status of IMP1 was elevated in the presence of SIRT6. Conclusion SIRT6 may regulate IGF2 though promoting the phosphorylation of IMP1 in 293T cells.

Key words: SIRT6, IGF2, IMP1, tumor regulator