Abstract: Objective To investigate the effect of different concentration of vinorelbine on apoptosis、telomerase activity and expression of human telomerase-reverse transcriptase gene （hTERT） in human epithelial ovarian cancer cells SKOV3. Methods Ovarian cancer cells SKOV3 were treated with vinorelbine under different concentrations. The cell proliferation was measured by Cell Counting Kit-8 （CCK-8） assay, and the cell apoptosis assay was detected by flow cytometry. The telomerase activity of SKOV3 cells was determined by TRAP-PAGE-silver staining; The mRNA expression of hTERT was performed by RT-PCR assay; Results: Vinorelbine could significantly inhibited the proliferation of SKOV3 cells、induce cell apoptosis（P＜0.01），reduce the telomerase activity and expression of hTERT mRNA（P＜0.01）, in dependen of a concentration-time manner. Conclusion：The detection of telomerase activity and the mRNA expression of hTERT might be vital for predicting the prognosis of patients with epithelial ovarian cancer.

Key words: Key words：epithelial Ovarian cancer, Vinorelbine, Apotosis, Telomerase activity