Abstract: Objective Study on the effect of isocorydine in human cervical cancer Siha cells xenografts in nude mice, to explore the inhibition mechanism of isocorydine in cervical carcinoma. Methods Establishment of human cervical cancer Siha cells subcutaneous xenografts model in BALB/c (nu/nu) nude mice. When the average diameter of the transplanted tumor≥0.5cm, mice were randomly assigned into control group and experimental group. In experimental group, the model was administered by intraperitoneal injection different doses of isocorydaline. After 4 weeks, the tumor tissues were removed, the histopathological changes of the tumor were observed by HE staining, and the expression of proteins in the tumor tissue were observed by immunohistochemical staining and Western blot. Results Compared with the control group, the tumor volume of experimental group was significantly decreased (P <0.05); the cell morphology can be transformed from stem cells to epithelioid cells, and the expression of E-cadherin was significantly increased while the expression of HPV16E6 and vimentin was decreased. Conclusions Isocorydine may inhibit the development of cervical cancer by inhibiting the expression of E6 protein, and EMT-related signaling pathway.

Key words: Isocorydine, Siha cells, xenografts, Protein expression