Abstract: Objective Using In Utero Electroporation technique to study the effects of miR-130b on the neuronal migration in the developing embryonic mice cortex. Methods Pregnancy 15.5 KM mice were selected and plasmid of miR-130b was injected into the lateral ventricle of the embryonic brain. In 2 days after eletroporation, mouse embryos were collected and cut into frozon coronal slices, then surveyed the neuronal migration under the fluorescence microscope. Results The neuronal migration rates were higher in miR-130b overexpression embryonic cortex, of which 75.1% eletroporated neurons migrated into MZ, compared with the 22.1% in control group. On the contrary, only 7.9% eletroporated neurons stayed in the VZ/SVZ in the experimental group, compared with the 69.3% in control group. Conclusions miR-130b accelerates the neuronal migration in the developing embryonic mice cortex, its regulational role is worth studying.

Key words: miR-130b, neurodevelopment, neuronal migration