Abstract: Objective To validate the expression level and the role of ENST00000460164 in luminal A breast cancer. Methods The expression level of ENST00000460164 in breast cancer tissues was detected by RT-qPCR. pll3.7-ENST00000460164-shRNA and empty vector，pll3.7，were transfected into MCF-7 cells. Cell cycle was measured by flow cytometry. Western blot was used to detect the expression of p16INK4A and cyclinD1. Results ENST00000460164 was highly expressed in luminal A breast cancer tissues compared to the adjacent non-cancer tissues. The knockdown of ENST00000460164 results in the G1 cell-cycle arrested, cyclin D1 downregulated and P16INK4A upregulated in MCF-7 cells. Conclusions ENST00000460164 is overexpressed in luminal A breast cancer. ENST00000460164 may control G1/S transition by regulating p16INK4A or cyclin D1 expression.

Key words: luminal A breast cancer, lncRNA ENST00000460164, cell cycle