Abstract: Objective To explore the effect of HIPK2 on apoptosis of human kidney tubular epithelial cells (HKC) induced by cisplatin. Methods Apoptosis of HKC cells was induced by cisplatin and the expression of HIPK2 was detected by real-time PCR and western blot. Two HIPK2 siRNAs were designed according to gene sequence of HIPK2 and cell lines with HIPK2 knockdownwere established through transfecting the HIPK2 siRNAs into HKC cells by liposome. The expression of HIPK2 mRNA and protein were detected by real-time PCR and western blot after induced by cisplatin. Then cell apoptosis was detected by Annexin V/PI after the HIPK2-knockdown cells were treated with cisplatin. Moreover, the expression of pro-apoptotic protein bax was detected by western blot after HIPK2 was knockdown. Results The expression level of HIPK2 mRNA and protein were down-regulated obviously on the process ofHKC apoptosis whichinduced by dose-dependent cisplatin (p<0.05). The transfection of siRNA can significantly reduce the expression of HIPK2 mRNA and protein in HKC (p<0.05), which will promote the HKC cells apoptosis induced by cisplatin. Conclusions HIPK2 can suppress the HKC cells apoptosis induced by cisplatin.

Key words: HIPK2, HKC, apoptosis, cisplatin