Abstract: Objective To investigate the impact of5-Fluorouracil (5-FU) and cisplatin on micrRNA-449b (miR-449b) expression in human hepatocellular carcinoma (HCC) and elucidate the molecular mechanism of 5-FU and cisplatin inhibiting the migration of HCC cells. Methods Real-time qPCR analysis was conducted to determine the expression of miR-449b in 50 HCC tissues. Real-time PCR assay was performed to detect the expression of miR-449b in HCC cells with 5-FU and cisplatin treatment. The migration of HCC cells with the overexpression of miR-449b was determined by wound-healing assay; Rescue assay was employed to investigate the correlation between 5-FU&cisplatin, miR-449b and the migration capacity of HCC cells; The putative targets of miR-449b were predicted and validated using target prediction programs and immunoblots. Results The expression of miR-449b decreased in HCC tissues (P<0.0001). MiR-449b expression increased in HCC cells upon the treatment of 5-FU and cisplatin (P<0.001). The overexpression of miR-449b inhibited the migration of HCC cells (P<0.001). Rescue assay revealed that inhibition of miR-449b to prevent 5-FU and cisplatin induction resulted in suppressed migration in SMMC7721 cells (P<0.05). Catenin-δwasa functional target of miR-449b. Conclusions 5-FU and cisplatin inhibit the migration of HCC cells at least partly via inducing the expression of miR-449b.

Key words: Key words: miR-449b, 5-FU, cisplatin, human hepatocellular carcinoma