Abstract: Objective To study the effects of Ceftriaxone on the seizures and the expression of glutamate transporter (GLT-1) in kainic acid (KA) epilepsy model. Methods Firstly, a chronic spontaneous seizure mouse model was established by unilateral hippocampal injection of KA and monitored by vEEG technique to record seizures. The experimental group received intraperitoneal injection of Cef 200 mg/(kg?d) and the control group received normal saline. Several indicators such as seizure frequency, interictal spike waves and histological phenotypes were used to evaluate the function of Cef. Then we use the western blot to detect the effect of expression for GLT-1. Results Unilateral hippocampal injection of KA 200ng successfully established the mesial temporal lobe epilepsy model. Cef can reduce the seizures from 2.145 times / day to 1.597 times / day, decreased by 31.2% with a statistical significance (p<0.05). Cef treatment did not significantly enhance the expression of GLT-1which test by Western blot. Conclusion Intraperitoneal injection of Cef partially inhibit the seizures of KA model, but the expression of GLT-1 in hippocampus was not enhanced. It is suggest that ceftriaxone may inhibit seizures through other mechanisms.

Key words: Key words: Ceftriaxone, Kainic acid-induced mesial temporal lobe epilepsy, Glutamate transporter GLT-1